【摘要】 对比了3种固相萃取柱(HLB柱、C18柱和PEP柱)对复杂乳品基质中目标物检测的影响,并建立了同时测定乳品中四环素类、β-内酰胺类及氯霉素等8种抗生素残留的固相萃取/高效液相色谱法。结果表明:HLB固相萃取柱的洗脱及净化效果较好,适用于复杂乳品基质的洗脱净化。在优化条件下,8种抗生素在5.0~100.0μg/L浓度范围内的线性关系良好(r2>0.999),检出限为0.09~0.84μg/kg,定量下限为0.32~2.23μg/kg。8种抗生素在不同加标浓度下(5,10,30μg/kg)的回收率为73.2%~93.3%,相对标准偏差(n=5)为1.4%~3.6%。研究结果表明,该方法适用于复杂乳品基质中8种不同种类抗生素的同时检测。更多还原

【Abstract】 The purified performance of three different types of solid-phase extraction columns,including HLB column,C18 column and PEP column,was investigated in this study. And based on that,a solid phase extraction / high performance liquid chromatography was developed for the simultaneous determination of 8 antibiotic residues in dairy. The result indicated that the HLB column showed an excellent elution and purification effect,and it was applicable for the dairy matrix purification. Under the optimal conditions,the calibration curves of eight antibiotics were linear in the concentration range of 5. 0-100. 0 μg / L with correlation coefficients( r2) more than 0. 999. The limits of detection( LOD) and limits of quantitation( LOQ) of 8 antibiotics were in the range of 0. 09-0. 84 μg /kg and0. 32-2. 23 μg / kg,respectively. The recoveries of target compounds at spiked concentration levels of 5,10 and 30 μg / kg ranged from 73. 2% to 93. 3%,with relative standard deviations( n = 5) of1. 4%-3. 6%. The developed method was suitable for the analysis of antibiotic residues in the complex dairy matrix.更多还原