【摘要】 目的 分析临床实验室常用的三种检测梅毒血清抗体方法的结果,为其预防和控制提供依据。方法 回顾性分析2013~2014年期间70829例住院、门诊及体检人群梅毒血清学检测结果,采用梅毒螺旋体特异性抗原血清试验化学发光微粒子免疫技术(TP—CMIA)初筛,明胶颗粒凝集试验(TPPA)进行验证,采用快速血浆反应素试验(RPR)测定阳性血清抗体滴度。结果 TP-CMIA初筛阳性1522例(阳性率为2.15%),TPPA阳性1401例(阳性率为1.98%),其中男性704例(50.25%),女性697例(49.75%),男女阳性率差异有统计学意义(X~2=4.65,P=0.031);男女阳性检出高发年龄段也呈明显差别,男性以61~70岁年龄组最多,女性以41~50岁年龄组最多。RPR检测抗体阳性541例,其中男性为270例,女性为271例,效价>1:8为80例(14.79%),<1:8为461例(85.21%),RPR阳性感染患者高发年龄段为61~70岁96例(17.74%),其次是71~80岁91例(16.82%),最低年龄段为1~10岁3例(0.55%)。结论 梅毒血清抗体阳性检出存在性别差异,男性高于女性,阳性检出高发年龄集中在60~70岁段,并且男女阳性检出高发年龄段有所不同。应根据梅毒检出人群的流行病学特征,制定相应的防控措施。梅毒血清学检测需要多种方法结合,减少误诊和漏诊。更多还原

【Abstract】 Objective To analyze the detection results of syphilis antibody with three methods frequently used in the clinical laboratory so as to provide a favorable basis for prevention and control of syphilis.Methods Among hospitalized patients,outpatients and health examination people,70 829 cases of the detection results of syphilis were retrospectivelyanalyzed from2013 to 2014 by Treponema pallidum-Chemiluminescence Microparticle Immuno Assay(TP-CMIA) to screen,Treponema Pallidum Particles Agglutinate(TPPA) to test and verify,Rapid Plasma Regain test(RPR) to determine antibody titre of positive serum.Results There were 1 522 positive cases by TP-CMIA screening(positive rate was 2.15%),1 401 positive cases by TPPA verification(positive rate was 1.98%),among which there were 704 males(50.25%) and 697 females(49.75%).There was statistical significance between the positive rates of males and females(x~2=4.65,F=0.031).There exist striking differences on the peak age for positive rates of males and females.The peak age for males was 61 — 70 years group,and that for females was 41 — 50 years group;there were 541 antibody-positive cases by RPR,among which there was270 males and 271 females,whose titre >1:8 were 80 cases(14.79%) and whose titre <1:8 were 461 cases(85.21%).The peak age for the patients with RPR positive infection was 61~70 years,amounting to 96 cases(17.74%),followed by71~80 years up to 91 cases(16.82%).The minimum age group was 1 —10 years,altogether 3 cases(0.55%).Conclusion There were gender differences in the positive test of syphilis serum antibody,and males were significantly higher than females.The peak age for positive rate was 60~70 years group,and that.of males and females is different.Prevention and control measures should be formulated according to epidemiological characteristics of patients infected with syphilis.A variety of metbods needs to be combined to perform syphilis serum detection in an effort to reduce the misdiagnosis and missed diagnosis.更多还原