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泡菜生香酵母的分离鉴定与挥发性香气成分分析
Isolation,identification and volatile compound analysis of an aroma-producing yeast from kimchi
【摘要】 [目的]研究特定生香酵母的挥发性香气,提升泡菜发酵的香气质量。[方法]从川南农家泡菜中筛选出一株生香酵母Y7,依据形态学和26S r DNA法鉴定该菌,并采用HS-GC-MS的方法分析其挥发性香气。[结果]系统发育树结果表明Y7与Kazachstania barnettii有高达99%的可信度。其挥发性香气成分有9种,主体挥发性香味是乙酸乙酯、丁酸乙酯、2-甲基-1-丙醇、3-甲基丁醇、3-甲基-3-丁烯-1-醇和苯乙醇,尤其是苯乙醇相对含量高达24.225%。[结论]为Kazachstania barnettii应用在泡菜发酵增香奠定基础。
【Abstract】 [Objective]Study specific aroma volatile aroma of yeast and enhance aroma quality of fermented kimchi. [Methods]A strain of aroma-producing yeast named as Y7 was isolated from south of Sichuan area. Morphological characteristics and 26 S r DNA sequence analysis to be used identified. HS-GC-MS method for the analysis of volatile aroma. [Results]Phylogenetic results showed that Y7 and Kazachstania barnettii had up to 99% confidence level. The aroma components had nine kinds,included ethyl acetate,ethyl butyrate,2-methyl-1-propanol,3-methyl-3-buten-1-ol,phenylethyl alcohol. In particular,the relative amounts of phenylethyl alcohol up to24. 225%. [Conclusion]The strain Y7 holds basis role for enhance aroma in kimchi ferment.
【Key words】 the kimchi from south of Sichuan; aroma-producing yeast; identification; volatile compound;
- 【文献出处】 生物技术 ,Biotechnology , 编辑部邮箱 ,2015年01期
- 【分类号】TS255.5;O657.63
- 【被引频次】13
- 【下载频次】418