【摘要】 目的建立一种同时测定蜜饯中7种常见合成色素(柠檬黄、苋菜红、胭脂红、日落黄、亮蓝、诱惑红、赤藓红)的固相萃取-高效液相色谱(solid-phase extraction-high performance liquid chromatography,SPE-HPLC)分析方法。方法样品经水提取后,采用Anpelclean PA聚酰胺SPE小柱净化,氮吹后定容,以甲醇和20mmol/L乙酸铵为流动相,通过Waters公司XBridge C18柱梯度洗脱分离,在特异性波长下进行测定。结果 7种常见合成色素在0.50~10.0μg/m L范围内峰面积与其实际浓度线性相关性良好(r2≥0.9987),在0.5、2.5、5.0mg/kg三个加标水平上回收率在74.3%~97.5%之间,RSD在0.47%~4.80%之间。检出限在0.02~0.10 mg/kg之间,定量限在0.06~0.30 mg/kg之间。结论本方法灵敏度高,分离效果好,精密度好,准确度高,适用于蜜饯中7种常见合成色素的测定。更多还原

【Abstract】 Objective To establish a method for simultaneous determination of 7 kinds of synthetic pigments(lemon yellow,amaranth,ponceau 4r,sunset yellow,coomassie brilliant blue,allura red and erythrosine) in preserved fruits with solid-phase extraction-high performance liquid chromatography(SPE-HPLC).Methods The homogenized samples were extracted by water,purified by Anpelclean PA-SPE column,and adjusted to constant volume after nitrogen blowing.HPLC analysis was conducted using methanol and 20 mmol/L ammonium acetate as mobile phase with a xbridge-C18 column from Waters company for gradient elution and separation.Specific wavelengths were applied for quantitative detection of the synthetic pigments in the samples.Results There was a good linearity between peak areas and concentrations of 7 kinds of synthetic pigments in the concentration range of 0.50~10.0 μg/m L(r2≥0.9987).The recovery rates fell in the scope of 74.3%~97.5% and RSDs ranged from 0.47% to 4.80% under the standard addition levels of 0.5,2.5 and 5.0 mg/kg.The detection limits were between 0.02 and 0.10 mg/kg and the quantification limits between 0.06 and 0.30 mg/kg.Conclusion The method has high sensitivity,good separation effect,good precision and high accuracy,thus,it’s suitable for the simultaneous determination of 7 kinds of common fruit pigments in preserved fruits.更多还原