【摘要】 目的探讨通解口服液对多器官功能障碍综合征(MODS)大鼠血清和回肠组织中IL-6、TNF-α的影响。方法将48只雄性SD大鼠随机分为空白组、模型组、阳性组、低剂量组、中剂量组和高剂量组,共6组,每组8只。各组均预先灌胃给予相应的干预措施,2天后,除空白组外,其余各组舌下静脉注射8μg/g脂多糖(LPS)复制MODS大鼠模型。观察大鼠生命体征(心电图、体温、呼吸幅度和频率)、血清指标(AST、ALT、CK、Cr)及血清和回肠组织IL-6、TNF-α含量。结果与空白组比较,模型组大鼠心率、体温、呼吸频率、血清指标(AST、ALT、CK、Cr)及血清和回肠组织TNF-α、IL-6含量差异有统计学意义(P<0.05,P<0.01)。与模型组比较,阳性组大鼠心率、体温差异有统计学意义(P<0.05);低、中、高剂量组大鼠心率差异有统计学意义(P<0.05,P<0.01)。与模型组比较,阳性组呼吸频率、血清指标及回肠组织中IL-6含量差异有统计学意义(P<0.05,P<0.01);高剂量组呼吸频率、血清指标、血清及回肠组织中TNF-α、IL-6含量差异有统计学意义(P<0.05,P<0.01);中剂量组血清指标、血清及回肠组织IL-6含量差异有统计学意义(P<0.05,P<0.01);低剂量组Cr水平差异有统计学意义(P<0.01)。与低剂量组比较,高剂量组血清ALT、AST、TNF-α、IL-6及回肠组织中IL-6含量,中剂量组的血清ALT含量差异有统计学意义(P<0.05,P<0.01)。与阳性组比较,低剂量组AST、ALT含量,高剂量组血清及回肠组织中IL-6含量差异有统计学意义(P<0.05,P<0.01)。结论通解口服液可有效降低脂多糖诱导的MODS大鼠血清及回肠组织中IL-6、TNF-α水平,且与其剂量具有相关性。更多还原

【Abstract】 Objective To investigate the effect"Tongjie Oral Solution"on IL-6 and TNF-α in serum and the ileum of rats with multiple organ dysfunction syndrome( MODS). Methods Fourty-eight male SD rats were randomly divided into 6 groups: control group,model group,positive control group,low dose treatment group,medium dose treatment group and high dose treatment group,with 8 rats in each group. Intragastric administration in advance,2 days later,intravenous inject Lipopolysaccharide( LPS) of 8mg / kg to build rat models. The vital signs such as electrocardiogram,temperature,breathing extent and respiratory rate; serum indexes such as AST,ALT,CK and Cr; levels of IL-6 and TNF-αin the serum and ileum were detected. Results There were significant differences in the heart rate,temperature,respiratory rate,serum indexes( contents of AST,ALT,CK and Cr in the serum),and contents of TNF-α and IL-6 between the blank group and model group( P < 0. 05,P < 0. 01). There were the significant differences in the heart rate and temperature between the model group and positive control group( P < 0. 05); There were the significant difference in the heart rate among the low-,medium-and high-dose treatment groups( P < 0. 05,P < 0. 01). There were significant differences in the respiratory rate,serum indexes and IL-6 level in the ileum between the model group and positive control group( P < 0. 05,P < 0.01); there were significant differences in the respiratory rate,serum indexes,TNF-α level and IL-6 level in the serum and ileum between the model group and high-dose treatment group( P < 0. 05,P < 0. 01); there were significant differences in the serum indexes,IL-6 in the serum and ileum between the model group and medium-dose treatment group( P < 0. 05,P < 0. 01); There was a significant difference in the Cr level between the model group and low-dose treatment group( P < 0. 01). There was a significant difference in the levels of ALT,AST,TNF-α and IL-6 in the serum and IL-6 in the ileum between the low and high-dose treatment groups,and there was a significant difference in the ALT level between the low and medium-dose treatment groups( P < 0. 05,P < 0. 01). There were significant differences in the contents of AST and ALT bwteen the positive control group and low-dose treatment group,and with significant differences in the IL-6 of the serum and ileum between the positive control group and high-dose treatment group( P < 0. 05,P < 0. 01). Conclusion "Tongjie Oral Solution "caneffectively reduce the levels of IL-6 and TNF-α in both serum and ileum of rats with MODS reduced by lipopolysaccharide. The curative effect was markedly coincident with the dosage of the solution.更多还原