【摘要】 目的 :分析2010年江苏省O1群霍乱疫情分离株病原学特征,为霍乱疫情分析及临床治疗提供实验室依据。方法 :对2010年江苏省O1群霍乱疫情分离株进行毒力基因检测、ctx B基因序列分析、抗生素敏感性实验、脉冲场凝胶电泳分子分型分析。结果:2010年江苏省O1群霍乱疫情分离株均携带毒力基因ctx A、ace、zot、tox R、tcp I、omp U、rtx C、tcp AEL、hly AEL,且CTXB氨基酸序列为古典型;对庆大霉素、诺氟沙星、环丙沙星100%敏感,对复方新诺明、链霉素100%耐药,且均为多重耐药株;除VC201014外,其余14株菌脉冲场凝胶电泳图谱相似度达100%。结论:2010年9月初至10月初,发生在江苏省徐州、淮安、宿迁、南京4个地市的O1群霍乱疫情可能是有关联的暴发流行,2010年8月底发生在连云港的O1群霍乱疫情是一起散发疫情,与上述四地的霍乱疫情可能没有流行病学关联,所有疫情的病原均为非典型埃尔托型霍乱弧菌,庆大霉素、诺氟沙星、环丙沙星可作为临床治疗的首选药物。更多还原

【Abstract】 Objective:To analyze the molecular characteristic and antibiotic resistances of Vibrio cholerae O1 stains in Jiangsu province in 2010,and provide laboratory evidence for the clinical analysis and treatment of cholera. Methods:V. cholerae O1 strains in Jiangsu province in 2010 were analyzed by virulence genes,sequence analysis of ctx B gene,antibiotic resistances and PFGE. Results:All strains were positive for ctx A,ace,zot,tox R,tcp I,omp U,rtx C,tcp AEL,hly AEL. They were also identified as harboring the classical ctx B genotype based on amino acid residue substitutions. They exhibited fully sensitivity to gentamicin,norfloxacin and ciprofloxacin,while they were all resistant to sulphamethoxazole / trimethoprim,streptomycin. The PFGE profiles of Not I showed a single banding pattern among 15 isolates,except VC201014. Conclusion:A single clone of a V. cholerae strain was responsible for the epidemic caused by V.cholerae O1 strains in Xuzhou,Huaian,Suqian,Nanjing during early September to late October in 2010,while there was another clone which was responsible for the epidemic in Lianyungang in late August in 2010. The bacterium type of V. cholerae causing the epidemics belonged to the atypical EL Tor variant. Gentamicin,norfloxacin and ciprofloxacin may be used for the treatment of this type of cholera.更多还原