【摘要】 野猪和地方猪养殖过程中仔猪营养性腹泻率高,严重地影响了养殖的经济效益。本研究通过PCR扩增和DNA测序,检测到猪乳糖酶基因(LCT)5’非编码区增强子和启动子多态性位点;通过双荧光报告系统实验,确定了LCT多态性位点调控基因的表达;经统计分析,LCT多态性与仔猪乳糖消化性状相关显著。通过饲养试验,筛选了抗腹泻饲料添加剂。上述研究形成了抗野猪和地方猪仔猪营养性腹泻的综合配套技术,为后续推广应用奠定了基础。更多还原

【Abstract】 High nutritional diarrhea rate in boar and local piglets affected the economic benefits seriously. In this study, the polymorphism loci of pig lactase(LCT) at 5’ non-coding enhancer and promoter was detected by PCR and DNA sequencing. And confirmed the regulating genes expression of LCT polymorphic loci through a experiment with dual luciferase report system. The correlation was significant between the polymorphism of LCT and piglets lactose digestive traits with statistical analysis. Then selected the anti-diarrheal feed additive by feeding trials.The study above formed an integrated technology for controlling nutritional diarrhea in boar and local piglets and laid the foundation for the subsequent application.更多还原