【摘要】 目的探讨白藜芦醇(Res)对脂多糖(LPS)诱导的破骨前体细胞Raw 264.7细胞系释放炎性细胞因子的抑制作用。方法采用LPS刺激Raw 264.7细胞构建炎症模型,采用抗酒石酸酸性磷酸酶(TRAP)染色鉴定细胞,采用MTT检测Res对Raw 264.7细胞的毒性影响,免疫荧光双标以及反转录聚合酶链反应(RT-PCR)方法检测不同浓度Res(1μmol/L和5μmol/L)对细胞炎性因子:肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)与细胞炎性蛋白酶:诱导型一氧化氮合酶(i NOS)和环氧合酶-2(COX-2)、炎性信号蛋白核因子-κB(NF-κB)蛋白与mRNA的表达变化。结果不同浓度的Res(1μmol/L和5μmol/L)在翻译水平和转录水平上明显抑制了LPS诱导的细胞炎性蛋白酶i NOS和COX-2表达,同时了抑制细胞炎性因子IL-1β与炎性信号蛋白NF-κB的上调。结论 Res可能通过NF-κB调控LPS诱导的Raw 264.7细胞炎性细胞因子的释放进而抑制破骨前体细胞的激活,进而具有抗骨质疏松的作用。更多还原

【Abstract】 Objective To investigate the inhibiting effects of resveratrol( Res) on the expression of potentially inflammatory cytokines by cultured osteoclast precursor Raw 264. 7 cells stimulated with lipopolysaccharide( LPS).Methods Inflammatory cell model was established by LPS-stimulated Raw 264. 7 cells. The Raw 264. 7 cell identification test was measured by tartrate resistant acid phosphatase( TRAP) staining. The cells were treated with Res( 1 μmol / L and5 μmol / L) prior to LPS( 5 mg / L) exposure. The effects on the mRNA and protein levels of inflammatory enzymes,inducible nitric oxide synthase( i NOS) and cyclooxygenase-2( COX-2),and inflammatory cytokines,tumor necrosis factor-α( TNF-α),and interleukin-1β( IL-1β),inflammatory signaling proteins nuclear factor-κB( NF-κB) were analysed by reverse transcription-polymerase chain reaction( RT-PCR) and double-immunofluorescence labeling assay. The effects of Res on cytotoxicity determination of Raw 264. 7 cells were measured by MTT assay. Results LPS-induced i NOS,COX-2 and NF-κB protein and mRNA expression levels were significantly decreased by Res. Res had an effect on the expression of TNF-α,IL-1β through transcriptional and translational inhibition. Conclusion The inhibitory effects of Res on LPS-induced inflammatory mediators in osteoclast precursor cells exert functions on its anti-osteoporosis through NF-κB.更多还原