【摘要】 [目的]该研究旨在探索番茄NAC转录因子与抗低温的关系。[方法]以番茄幼苗为试材,采用RT-PCR方法克隆NAC1基因N端保守域,并利用RT-q PCR技术检测该基因在4℃低温l处理下的表达情况。[结果]在番茄根、茎、叶中NAC1基因均能表达,表达量随低温处理时间的变化而发生相应的变化。在番茄的不同器官中NAC1表达水平也有所不同,根中的表达水平整体高于茎和叶片中。[结论]该研究为进一步研究番茄NAC1基因的生物学功能奠定理论基础。更多还原

【Abstract】 [Objective] This study aimed to explore the relationship between NAC tran scription factor and resistance to low temperature in tomato. [Method] Tomato seedlings were used as experimental materials to clone the N-terminal conserved domain of NAC1 gene with RT-PCR and investigate the expression patterns o NAC1 gene under 4 ℃ with real-time quantitative PCR(RT-q PCR). [Result] NAC1 gene was expressed in roots, stems and leaves of tomato, but the expression leve varied with the change of treatment duration. Moreover, the expression levels o NAC1 varied among different organs of tomato. The expression level of NAC1 in roots was much higher than that in stems and leaves. [Conclusion] This study laid a theoretical foundation for further investigation of the biological function of NAC1 gene in tomato.更多还原