【摘要】 目的比较哮喘患者与健康受试者尿液中水通道蛋白2(aquaporin 2,AQP2)的含量,探讨哮喘不同临床控制水平AQP2含量的差异及与炎症介质的相关性。方法检测60例哮喘患者及21例健康受试者(正常对照组)尿液中AQP2含量;CBA技术检测血浆白介素(IL)-4、IL-6、肿瘤坏死因子(TNF)-α及干扰素(IFN)-γ水平。结果哮喘未控制组(ACT在19分以下)及部分控制组(ACT 20~24分)患者尿液AQP2水平均较正常对照组增高(P<0.01),且未控制组增高明显,达(174.28±40.81)pg/mL;血浆IL-4含量在哮喘未控制组最高(1.10±0.25)pg/mL,与正常对照组及哮喘部分控制组比较,差异有统计学意义(P<0.01);血浆IL-6含量在哮喘未控制组最高,但与正常对照组及哮喘部分控制组比较,差异无统计学意义(P=0.058);TNF-α含量哮喘组高于对照组,差异有统计学意义(P<0.05)。尿液AQP2与血浆IL-4、IL-6均有相关性(P=0.049,P=0.010),IL-4与IFN-γ相关(P=0.019),IL-6与TNF-α有相关性(P=0.010);IL-10与IFN-γ相关(P=0.005)。结论哮喘患者尿液AQP2水平增高,且与血浆炎症介质IL-4、IL-6相关,尿液AQP2在支气管哮喘发病机制中的作用值得进一步研究。更多还原

【Abstract】 Objective To compare the urine aquaporin 2(AQP2)levels between asthmatic patients and healthy participants,explore the AQP2 levels in patients with asthma of different clinical control levels and its relationship with inflammatory factors.Methods Urine AQP2 levels were examined by ELISA in 60 patients with asthma and 21 healthy participants;plasma interleukin(IL)-4,IL-6,tumor necrosis factor(TNF)-αand interferon(IFN)-γlevels were determined by by CBA method.Results The levels of urine AQP2 in clinical uncontrolled asthma group(ACT below 19)and partially controlled asthma group(ACT 20~24)were significantly higher than that in the healthy control group(P<0.01),and the level in the uncontrolled group was(174.28±40.81)pg/mL.The plasma IL-4level in uncontrolled group was(1.10±0.25)pg/mL,which was significantly higher than those in the other two groups(P<0.01).The plasma IL-6level in uncontrolled asthma group was the highest but showing no significant difference when compared with the other two groups(P=0.058).The TNF-αlevel in asthma patients was significantly higher than that in healthy controls(P<0.05).Urine AQP2 was found significantly correlated with plasma IL-4and IL-6levels(P=0.049,P=0.010);plasma IL-4level was significantly correlated with IFN-γlevel(P=0.019);IL-6was significantly correlated with TNF-αlevel(P=0.010);and IL-10 was significantly correlated with IFN-γlevle(P=0.005).Conclusion Urine AQP2 level of asthma patients is increased,and it is significantly correlated with plasma levels of inflammatory factors IL-4and IL-6.The role of AQP2 in the pathogenesis of bronchial asthma is worth further studying.更多还原