【摘要】 目的分析NPC1L1基因1 735C>G多态性分布特征及其与血脂、肥胖的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对409例汉族人群进行NPC1L1基因多态性分型,比较不同基因型间血脂水平、BMI的差异。结果 CC,CG,GG基因型的分布频率分别为39.40%、46.00%、14.60%;等位基因C和G频率分别为62.35%和37.65%。3种基因型组间的LDL-C、ApoB水平和BMI的差异有统计学意义(P<0.05),G等位基因携带者(GC/GG基因型)较CC型有更高的LDL-C、ApoB水平(P<0.05)。血脂谱和BMI与多种环境因素相关。结论 NPC1L1基因1 735C>G多态性与血脂水平和BMI相关,G等位基因可能是高LDL-C水平和肥胖的一个危险因素。更多还原

【Abstract】 Objective To analyze the distributional characteristics of NPC1L1 1 735C>G polymorphism and the relationship between serum lipid levels and obesity in Guangxi Han population.Methods Genotyping of the NPC1L1 1 735C>G polymorphism of 409 cases of Han was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis in all subjects.The relationship of different genotypes in blood lipid levels and obesity were evaluated.Results The frequency of CC,CG and GG genotypes was 39.40%,46.00% and 14.60%in Han.The frequency of C and G alleles was 62.35%and 37.65%in Han.The levels of BMI,LDL-C,ApoB was different among the three genotypes(P<0.05).The G allele carriers had higher serum LDL-C and ApoB levels than the G allele noncarriers(P<0.05).Serum lipid parameters and BMI were also correlated with some environmental factors.Conclusion The 1 735C>G polymorphism in NPC1L1 gene may be correlated with serum lipid profiles and obesity,the G allele might increase the risk of hyperlipemia and obesity.更多还原