【摘要】 目的探讨健脾化湿颗粒对腹泻型肠易激综合征(D-IBS)大鼠模型降钙素基因相关肽(CGRP)表达的影响。方法将60只Wistar大鼠分成对照组(n=10)和造模组(n=50),再将造模组大鼠分为5组:模型组、阳性对照组及健脾化湿颗粒低、中、高剂量治疗组,每组10只。采用束缚应激刺激2周加灌服番泻叶煎剂的方法建立D-IBS大鼠模型,采用免疫组化法检测各组大鼠小肠黏膜CGRP的表达情况。结果与对照组比较,模型组大鼠小肠黏膜CGRP阳性面积减少,差异有统计学意义(P<0.05);与模型组比较,各治疗组CGRP阳性面积增加,差异有统计学意义(P<0.05);与阳性对照组比较,健脾化湿颗粒中、低剂量治疗组CGRP阳性面积增加,差异有统计学意义(P<0.05),而与健脾化湿颗粒高剂量治疗组比较,差异无统计学意义(P>0.05)。结论健脾化湿颗粒可通过提高局部肠组织中CGRP水平而达到治疗目的。更多还原

【Abstract】 Objective To observe the effects of Invigorating Spleen and Eliminating Dampness Granules on calcitonin gene related peptide(CGRP)in rat models of diarrhea predominant irritable bowel syndrome(D-IBS).Methods 60 Wistar rats were randomly divided into the control group(n=10)and the constructing model group(n=50).Then,the constructing model group was randomly re-divided into the model group(n=10),positive control group(n=10),invigorating spleen and eliminating dampness Granules low,middle and high dose groups(n=10each).The D-IBS rat model was established by the restraint stress stimulation and gavage of folium sennae decoction for 2weeks.The expression of CGRP in small intestinal mucosal membrane in each group was detected by the immunohistochemical method.Results In the model group,the CGRP positive area was decreased,which showed the statistical difference compared with the control group(P<0.05);the CGRP positive area in various treatment groups was increased,which showed the statistical difference compared with the model group(P<0.05);in the invigorating spleen and eliminating dampness granules middle and low dose groups,the CGRP positive area was increased with the statistical difference compared with the positive control group(P<0.05),while without the statistical difference compared with the invigorating spleen and eliminating dampness granules high dose group(P>0.05).Conclusion Invigorating spleen and eliminating dampness granules achieves the therapeutical effect by increasing the level of CGRP in local intestinal tissue.更多还原