【摘要】 目的探讨肿瘤坏死因子(TNF-α)、细胞间黏附分子-1(ICAM-1)在梗阻性黄疸(OJ)肝细胞损伤中的作用,以及黄芪对OJ幼鼠肝细胞TNF-α及ICAM-1表达的影响及临床意义。方法将90只Wistar大鼠随机分为正常对照组、OJ组、OJ+黄芪(A)三组,OJ+A组给予黄芪注射液250mg/100g体质量腹腔注射,作用7d后应用放射免疫法检测各组血清TNF-α含量,免疫组织化学法检测肝细胞TNF-α、ICAM-1的表达。结果 1血清检测:ALT在OJ及OJ+A明显高于对照组,在OJ组明显高于OJ+A组;DBIL在OJ组与OJ+A无显著差别。2OJ组及OJ+A组血清TNF-α含量明显高于正常对照组;OJ组实验鼠血清TNF-α高于OJ+黄芪组;3TNF-α及ICAM-1在肝细胞的表达:OJ组及OJ+A组的表达较正常对照组明显增强,OJ组表达强于OJ+A组。结论本研究结果表明OJ大鼠出现肝功能损害,肝细胞TNF-α、ICAM-1表达增强;应用黄芪有助于减轻OJ所致的肝功能损害,对肝细胞有保护作用,其机制可能与降低肝细胞内TNF-α、ICAM-1表达有关。更多还原

【Abstract】 Objective To investigate the effects of Astragalus Iniection on expression of TNF-αand ICAM-1in Liver cells of rats with obstructive jaundice(OJ).Methods 90 Wistar rats were divided randomly into normal group,obstructive jaundice(OJ)group,OJ + astragalus(A)group.In OJ+A group,astragalus injection(250 mg/100 g body weight)were given for 7days.The content of TNF-αin serum was detected by radioimmunoassay,and the expression of TNF-αand ICAM-1in liver cells was detected by immunohistochemistry.Results 1 Serologic examination:The Level of serum TNF-αin OJ group and OJ+ A group were significantly higher than that in the normal group,P<0.01;The Level of serum TNF-αin OJ group was higher than that in the OJ+ A group,P<0.05;2The expression of TNF-αand ICAM-1in liver cells of OJ group and OJ+ A group were increased significantly when compared with the normal group.But in OJ+ A group,the expression of TNF-αand ICAM-1were lower than that in OJ group.Conclusion Results of this research demonstrated that the expression of TNF-αand ICAM-1in liver cells of rats with obstructive jaundice were increased significantly.Application of astragalus could decrease the expression of TNF-αand ICAM-1in liver cells.It was indicated that astragalus could play aprotective effect on liver cells in rats with obstructive jaundice.Application of astragalus could conduce to ameliorate hepatic damage in rats with obstructive jaundice,the mechanism might be related to the decreased expression of TNF-αand ICAM-1in liver cells.更多还原