【摘要】 以欧美杂种山杨嫩枝插穗为实验材料,进行扦插不定根生成过程中插穗形态和解剖学观察,通过Solexa高通量测序对扦插后6和12 d插穗不定根生成部位进行转录水平上的检测分析。插穗解剖观察发现扦插后6 d为根原基的诱导期,12 d为不定根的伸长生长期,对插穗不定根2个时期高通量测序获得差异表达基因2 328个。对筛选出的显著差异表达基因进行功能注释、分类和KEGG及pathway分析,确定2个山杨样本中差异表达基因参与的生化代谢和信号转导途径。更多还原

【Abstract】 We used Populus tremula × P. tremuloides softwood cuttings as experimental material, and observed morphological and anatomical changes of the cuttings during the rooting process of adventitious roots. Solexa high-throughput sequencing was performed for the parts of cuttings where adventitious roots generated 6 days and 12 days after cutting( DAC) at transcriptional level. Anatomical observation showed that the induction stage of the adventitious root primordia was 6 days after cutting and the elongation stage of adventitious roots was 12 days after cutting. A total of 2 328 differentially expressed genes were obtained by the Solexa high-throughput sequencing. The significantly differential expression genes were classified after their functions annotation,and then the KEGG passway analysis was carried out. On this basis,the biochemical metabolic and signal transduction pathways of these differentially expressed genes were determined.更多还原