【摘要】 目的:建立岗梅感冒灵颗粒中3种有效成分（齐墩果酸、腺苷、蒙花苷）的含量测定方法。方法:采用HPLC法测定样品含量,色谱柱为Agilent柱（125 mm×4 mm,5μm）,流动相分别为乙腈-1%冰醋酸（88:12）、甲醇-水（8:92）、甲醇-2%冰醋酸（52:48）,检测波长分别为205,260,334 nm。流速为1.0 ml·min^-1,进样量5μl.柱温为25℃。结果:齐墩果酸、腺苷和蒙花苷分别在0.005⁰.06,0.001⁰.02,0.001⁰.02 mg·ml^-1范围内线性关系良好,r值分别为0.999 9,0.999 9和0.999 1;平均回收率分别为99.16%,98.30%,101.50%,RSD值分别为1.28%,1.99%,1.43%。结论:该方法操作简便、准确、重复性好,可用于岗梅感冒灵颗粒中3种有效成分的含量测定。更多还原

【Abstract】 Objective:To establish a method for the determination of three components（oleanolic acid,adenosine and linarin） in Gangmei Ganmaoling granules.Methods:The chromatographic separation was achieved on an Agilent column（125 mm×4 mm,5μm） with a mobile phase composed of aeetonitrile-1%acetic acid（88:12）,methanol-water（8:92） and methanol-2%acetic acid（52:48）,respectively.The detection wavelength was set at 205 ran,260 nm and 334 nm,respectively.The flow rate was 1.0 ml·min^-1and the column temperature was kept at 25℃.Results:Oleanolic acid,adenosine and linarin had a good linearity within the range of0.005-0.06 mg·ml^-1.0.001-0.02 mg·ml^-1 and 0.001-0.02 mg·ml^-1 with the correlation coefficients of 0.999 9,0.999 9 and0.999 1,respectively.The average recovery was 99.16%,98.30%and 101.50%,respectively.RSD was 1.28%,1.99%and1.43%,respectively.Conclusion:The method is proved to be simple and accurate with good repeatability.It can be used in the content determination of the three components in Gangmei Ganmaoling granules.更多还原