【摘要】 目的:建立同时测定大鼠口服旱莲草提取物后血浆中4种黄酮含量的HPLC法。方法:采用高效液相色谱法,色谱柱为Neocleodur 100-5 C18柱(250 mm×4.6 mm,5μm),流动相A为甲醇-0.2%磷酸溶液(50∶50),B为乙腈,梯度洗脱。流速为1.0 m L/min,检测波长为350 nm,柱温40℃。结果:木犀草素、芹菜素、野黄芩素和香叶木素在浓度为0.02~50μg/m L时与色谱峰面积比之间的线性关系良好,平均回收率为71.5%~90.2%。结论:该方法快速、简便、准确,可用于大鼠血浆中木犀草素、芹菜素、野黄芩素和香叶木素的HPLC测定及药动学研究。更多还原

【Abstract】 Objective: To establish an HPLC method for determination of four flavonoids in rat plasma after oral Ecliptae Herba extract. Methods: HPLC separation was carried out in a Neocleodur 100-5 C18column( 250 mm × 4. 6 mm,5 μm) with mobile phase A( consisting of methanol-0. 2% phosphoric acid = 50∶ 50) and mobile phase B( acetonitrile) with gradient elution at the flow rate of 1. 0m L / min. The detection wavelength was 350 nm and the column temperature was maintained at 40 ℃. Results: There were good linear relationships between concentration and peak area ratio of luteolin,apigenin,scutellarein and diosmetin in the ranges of 0. 02 ~ 50 μg /m L with the recovery rates of 71. 5% ~ 90. 2%. Conclusion: This method is rapid,accurate and reproducible for determination of luteolin,apigenin,scutellarein and diosmetin in rat plasma and study on pharmacokinetics of Ecliptae Herba extract.更多还原