【摘要】 目的:研究加味四逆汤保护免疫性肝损伤和T淋巴细胞在其中的作用。方法:将健康雌性NIH小鼠50只,随机分为5组:正常对照组、刀豆蛋白A(Con A)模型组、联苯双酯组(LB,150 mg·kg-1)、加味四逆汤高、低剂量(120,240 mg·kg-1)组。所有的小鼠均经尾静脉注射Con A(18 mg·kg-1)来建立急性免疫性肝损伤动物模型,在造模成功12 h后摘取小鼠眼球采血。通过流式细胞术测定全血中CD4+,CD8+T细胞亚群,并以酶联免疫(ELISA)法测定血清中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)的活性和肿瘤坏死因子-α(TNF-α),γ-干扰素(IFN-γ)和白介素-10的水平。结果:模型组小鼠血清ALT,AST活性和TNF-α,IFN-γ水平明显高于正常组(P<0.01),全血中CD4+,CD8+比率较正常对照组明显下降(P<0.05);与模型组比较,加味四逆汤高、低剂量组均能降低Con A介导的肝损伤小鼠血清中AST,ALT的活性(P<0.05),剂量组间差异无统计学意义;且能明显降低模型组血清中已升高的炎性细胞因子IFN-γ,TNF-α的含量(P<0.05,P<0.01),而提高肝损伤中已经降低的抗炎因子IL-10的活性和T细胞亚群CD4+,CD8+的比率(P<0.05)。结论:加味四逆汤对免疫性肝损伤具有保护作用,其作用机制可能通过调整T细胞亚群的活性、减少炎性细胞因子和增加抗炎细胞因子的释放有关。更多还原

【Abstract】 Objective: To observe protective effect of Modified Sini decoction on immunological liver injury in mice induced by concanavalin A( Con A) and the role of T lymphocyte subsets in which. Method: The mouse were randomly divided into normal saline( NS) group,Con A group,bifendate group( LB,150 mg·kg-1),Modified Sini decoction low,high dosage group( 120,240 mg·kg-1). All groups,except for the NS group,were given Con A aqueous solution( 18 mg·kg-1) via tail vein injection 1 hour after drug administration at d1 and d3,peripheral blood was collected 12 h after Con A was injected. The percentage of T lymphocyte subsets CD4+,CD8+cells were detected in plasma by a flow cytometer,using enzyme-linked imunosorbent assay( ELISA) for determination the serum cytokines: interferon-γ( IFN-γ),tumor necrosis factor alpha( TNF-α),interleukin( IL)-10 and activities of alanine aminotransferase( ALT),aspartate aminotransferase( AST).Result: The activity of AST and ALT of Modified Sini decoction treatment groups significantly dropped in mice of Con A-mediated liver injury; SND-treated groups could significantly reduced the liver injury model group had elevated inflammatory cytokines IFN-γ or TNF-α level( P < 0. 05,P < 0. 01); Modified Sini decoction groups could improve the liver injury model group had lowed T-cell subsets CD4+,CD8+rate( P < 0. 05).Conclusion: Modified Sini decoction is effective in protecting immunological liver injury in mice induced by Con A and this mechanism maybe closely associated with its action in regulating the T-cell subsets and in reducing the inflammatory cytokines.更多还原