【摘要】 目的:采用超声方法提取新疆紫草中紫草素,并以左旋紫草素为指标,建立HPLC测定紫草素的含量。方法:利用超声波协同甲醇提取40 min;色谱条件为Shim-pack CLC-ODS-18色谱柱(4.6 mm×250 mm,10μm),检测波长516 nm,流动相甲醇-水(85∶15),流速1.0 mL·min-1,柱温室温。结果:左旋紫草素进样量在0.127~1.143μg具有良好的线性关系,回归方程为Y=338 763X-198 347(r=0.999 8),平均回收率99.3%,RSD 1.18%,左旋紫草素超声法提取率是回流提取法的1.29倍。结论:经方法学验证,该实验所采用的实验方法重复性好、稳定、准确、可靠,可用于中药紫草药材中紫草素的含量测定。更多还原

【Abstract】 Objective: To develop a method for ultrasonic-assisted extraction and high performance liquid chromatography( HPLC) determination of shikonin in the roots of Arnebiae Radix. Method: Shikonin was extracted by ultrasonic-assisted-methyl alcohol method. The HPLC determination condition consisted of Shim-pack CLC-ODS-18( 4. 6 mm × 250 mm,10 μm) and shikonin was detected at 516 nm wavelength by using methyl alcohol-water( 85∶15) as the mobile phase,column temperature at room temperature and flow rate 1. 0 mL·min-1. Result: Shikonin had a good linearity relation in the range 0. 127-1. 143 μg( r = 0. 999 8),regressive equation were Y = 338 763 X-198 347. The average recovery was 99. 3%,RSD 1. 18%( n = 6). In contrast with refluxing process,the extraction efficiency of ultrasonic extraction was 1. 29 times. Conclusion: The methods of ultrasonic extraction and HPLC determination were proved to be accurate,reliable and repeatable. It can be used to determine natural shikonin content of Arnebiae Radix easily.更多还原