【摘要】 研究了药物对山女鳟水霉病病原的抑制及杀灭效果,即中草药抑制水霉菌丝生长和抗水霉制剂抑制孢子的生长。实验选用过氧化氢、甲醛等6种常用抗水霉制剂及地肤子、蛇床子等16种中草药单方和2种复方(T1、T2)分别采用孢子计数法和琼脂平板扩散法对水霉游动孢子及菌丝的体外抑菌效果进行研究。研究结果显示,过氧化氢和甲醛抑孢子的效果优于孔雀石绿,而检科Ⅰ号、亚硝酸钠和过氧乙酸的抑制效果不及孔雀石绿。石榴皮和复方T1能有效抑制菌株SN的菌丝生长;地肤子、肉桂、柠檬、苦参、大蒜、黄精、茴香和菖蒲对菌株几乎无抑菌作用;黄芩、虎杖、金银花、苦楝皮、复方T2、蛇床子、土槿皮和大黄均有较好的抑制作用。实验为合理的筛选有效抑山女鳟水霉菌药物及制定科学有效地抗霉剂使用方法提供理论依据。更多还原

【Abstract】 The paper aims to study the effects of drugs that inhibit and kill pathogens, including ChineseMedicinal herbs that inhibit hyphae growth and anti- Saprolegnia agents that inhibit zoospore. The authorschose 6 commonly used anti- Saprolegnia agents(such as hydrogen peroxide, formaldehyde et al.) and 16 Chinese medicinal herbs(such as Fructus kochiae Scopariae, Cnidium monnieri et al.) and 2 compoundprescriptions(T1 and T2), used the method of spore counted and agar plate to conduct the research. The resultsshow that hydrogen peroxide and formaldehyde inhibit spore better than malachite green, but the inhibitoryeffect of JiankeⅠ, sodium nitrite and peracetic acid are less than malachite green. The pomegranate rind andcompound T1 can effectively inhibit the hypha growth; Fructus kochiae Scopariae, Cinnamomum cassia Presl,Citrus limon, Radix Sophorae flavescentis, Allium sativum, Polygonatum sibiricum, Foeniculum vulgare and Acorus calamus have almost no inhibitory effect on the strain; Scutellaria baicalensis Georgi, Reynoutria japonica Houtt, Lonicera japonica Thunb, Cortex meliae, compound T1, Cnidium monnieri, Pseudolarix kamepferi Gord and Rheum palmatum have better inhibitory effect. The experiment provides theoretical basisfor screening the medicines which have the efficacious antibacterial activity on Saprolegnia in Oncorhynchus masou masou and developing scientific and effective utilization method of anti-Saprolegnia agents.更多还原