【摘要】 目的:研究以粉尘螨提取液致敏C57BL/6小鼠产生高水平IgE的小鼠哮喘动物模型的建立和评估。方法:C57BL/6小鼠随机分为对照组和哮喘组。哮喘组于实验第0、7、14天分别以粉尘螨提取液腹腔致敏,共3次,对照组以PBS代替。于实验第28天起,哮喘组小鼠以粉尘螨提取液滴鼻激发,连续7天,观察并记录小鼠哮喘发作情况;对照组以PBS代替。最后1次滴鼻24 h后对小鼠作肺泡灌洗液。进行灌洗液细胞计数和分类,肺组织病理学检测(HE染色),ELISA测定血清总IgE和重组粉尘螨二类变应原特异性IgE(rDer f 2-sIgE)含量,ELISA试剂盒测定支气管肺泡灌洗液(Bronchoalveolar Lavage Fluid,BALF)中IL-4和IFN-γ的含量。结果:哮喘组小鼠肺泡灌洗液中细胞总数和嗜酸性粒细胞较正常组极显著增加(P<0.01);肺组织可见明显的炎性细胞浸润;血清总IgE(P<0.001)和rDer f 2-sIgE(P<0.01)水平极显著升高;BALF中IL-4水平显著增高(P<0.05),而IFN-γ的水平极显著下降(P<0.01)。结论:使用多次腹腔致敏和滴鼻激发的方法可以建立产生高水平IgE的粉尘螨过敏性哮喘动物模型。更多还原

【Abstract】 Objective: To construct a mouse asthma model sensitized and challenged by Dermatohpagoides farinae extracts with high level of IgE. Methods: C57BL /6 mice were randomly divided into control group and asthma group. Mice in asthma group were sensitized by Dermatohpagoides farinae extracts through intraperitoneal injection at day point 0,7 and 14,PBS instead for the control group. On day 28,the mice of asthma group were challenged by intranasal Dermatohpagoides farinae extracts for seven consecutive days and the ones of control group were administrated by PBS instead. Observe and record the symptoms of asthma in each group. After the final allergen challenging,the total and sorting cell count of bronchoalveolar lavage fluid( BALF),histological examination,serum IgE and IL-4 and IFN-γ in BALF were carried out. Results: The number of total cells and eosinophils in BALF of asthma group was significantly higher than that in the control group( P < 0. 01). Inflammatory cells were obviously observed in the lung tissues of the asthma group. The level of total IgE( P < 0. 001) and rDer f 2-sIgE( P < 0. 01) in serum enhanced significantly. The level of IL-4 in BALF was significantly increased( P < 0. 05),while the INF-γ was decreased( P < 0. 01). Conclusion: It is possible to construct a mouse model of asthma through repeatedly sensitizing and challenging by Dermatohpagoides farinae extracts to product high level of IgE.更多还原