【摘要】 目的探讨丝裂原活化蛋白激酶(MAPK)家族成员p38MAPK、细胞外信息调节蛋白激酶(ERK1/2)在非小细胞肺癌(NSCLC)中的表达及临床意义。方法选取43例NSCLC的肿瘤组织及其癌旁正常组织作为对照。采用蛋白免疫印迹法检测p38MAPK、ERK1/2的表达情况.分析其与临床特征(包括性别、年龄、肿块大小、淋巴结转移情况、分化程度、组织类型以及病理分期)的关系,及其表达程度与预后的关系。结果与配对的正常肺组织比较,phospho-p38MAPK、phospho-ERK1/2在NSCLC组织中活化上调(均P<0.01)。43例患者中37例phospho-p38MAPK的表达平均增长2.95倍。39例phospho-ERK1/2表达平均增长3.07倍.差异均有统计学意义(均P<0.01)。ERK1/2的表达与各临床特征均无明显相关(均P>0.05),而p38MAPK的活化与肿瘤大小以及年龄相关(均P<0.01)。ERK1/2未活化组、中度活化组与高度活化组的生存曲线依次下移,p38MAPK高度活化组生存曲线明显降低。Cox比例风险回归模型分析显示,phospho-p38MAPK的高表达是预后不良的预测因素(P<0.05)。结论 p38MAPK与ERK1/2的活化表达可能在NSCLC的发生/发展过程中具有重要意义,其中p38MAPK的活化程度可辅助作为非小细胞肺癌的预后评估因素。更多还原

【Abstract】 Objective To examine the expression of phospho-p38 MAPK,phospho-JNK,and phospho-ERK1/2 in non-small cell lung cancer(NSCLC) and its clinicopathological significance.Methods Forty-three specimens of primary non-small cell lung cancer and paired normal lung tissue were collected.The expressions of phospho-p38 MAPK,phospho-ERK1/2 and phospho-JNK were detected with Western-blot analysis.The association between clinicopathological parameters and the expression of phospho-p38 MAPK and phospho-ERK1/2 protein was analyzed.Results Compared with corresponding normal lung tissue,the expression of phospho-p38 MAPK and phospho-ERK1/2 in NSCLC tissue were increased(P<0.01).In 37 cases,phospho-p38 MAPK expression were increased by average 2.95 times(P<0.01),while in 39 cases phospho-ERK1/2 expression were increased by 3.07 times(P<0.01).The expression of ERK1/2 was not correlated with clinicpathological parameters(P > 0.05).The activation of p38 MAPK was correlated with tumor size and ages of patients(both P<0.01).The expressions of ERK1/2 and p38 MAPK were negatively correlated with survival.Multivariate analysis revealed that the expression of phospho-p38 MAPK was an independent prognostic indicator for overall survival of patients(P=0.046).Conclusion ERK1/2 and p38 MAPK are activated in NSCLC,and the expression of phospho-p38 MAPK can help to predict the prognosis of NSCLC.更多还原