【摘要】 黑李溃疡病为近年来发生在浙江省黑李产区的一种严重病害,主要危害李树当年生、多年生枝和主干,引起枝条枯死和整株死亡,继而毁灭整个黑李果园。通过对黑李溃疡病典型症状样本的采集、病原菌的分离、纯化和致病性测定,明确引起该病的病原菌为一种细菌。通过对黑李溃疡病菌的菌体形态和培养特性观察,生理生化反应、脂肪酸分析、16S rDNA序列分析和种的特异性引物的检测分析,证明该病原细菌为树生黄单胞菌李变种Xanthomonas arboricola pv.pruni(Smith)Vauterin et al.。更多还原

【Abstract】 Black plum(Prunus americana) canker is a serious disease occurring in recent years in black plum plantation in Zhejiang Province. It mainly infects current-year shoots,perennial branches and trunks,causing the branches withered and the whole plant died,and finally destroyed the whole black plum orchard. In this study,we collected the typical symptom samples of this disease,isolated and purified the pathogen,and then the pathogenicity determination results showed that the pathogen causing black pulm canker was a kind of bacteria. The pathogenic bacteria was identified as Xanthomonas arboricola pv. Pruni(smith) Vauterin et al. based on the bacteria cell morphology,cultural characteristics,physiological and biochemical characteristics,analysis of fatty acid methylesters(FAME) and 16 S rDNA gene sequence.更多还原