【摘要】 目的体外观察赖氨匹林对转染并稳定表达连接蛋白32/26(Cx32/Cx26)的Hela细胞缝隙连接通讯(GJIC)功能和Cx32/Cx26蛋白表达水平的影响。方法不同浓度(0、1、5、10mmol·L-1)赖氨匹林作用于人宫颈癌Hela细胞24h后,以划痕标记/染料示踪技术(SL/DT)检测赖氨匹林作用Hela细胞48h后荧光黄在细胞之间传递的距离来评价其对缝隙连接(GJ)功能的影响;Western blot方法检测赖氨匹林对Cx32/Cx26蛋白表达的影响。结果 SL/DT检测显示赖氨匹林具有上调GJ功能的作用;Western blot显示赖氨匹林增加Cx32/Cx26蛋白表达水平。结论赖氨匹林抑制转染并稳定表达Cx32/Cx26的Hela细胞的增殖、上调GJ功能且这种作用可能与其增加Cx32/Cx26蛋白表达水平有关。更多还原

【Abstract】 Objective To investigate the effect of aspisol on gap junction(GJ)function in Hela cells transfected with connexin 32and connexin 26(Cx32/Cx26)plasmid and expression of Cx32/Cx26protein.Methods Hela cells were incubated with aspisol at various concentrations(0,1,5,10mmol·L-1)for24h.The GJ function of Hela cells treated with aspisol for 48hours was detected by scrape-loading dye transfer(SL/DT),and the effect on GJ function was evaluated by the transmission distance of lucifer yellow between cells.Western blotting was performed to detect the expression level of Cx32/Cx26increased by aspisol.Results SL/DT showed that aspisol upregulated the function of GJ of Hela cells.Western blotting showed that aspisol increased the Cx32/Cx26protein expression.Conclusion Aspisol can inhibit proliferation of Hela cells transfected with connexin 32and connexin 26plasmid which stably expressed Cx32/Cx26and upregulate GJ function.These effects are possibly associated with the increase of Cx32/Cx26expression induced by aspisol.更多还原