【摘要】 目的:研究芦丁对高糖模型大鼠肾小球系膜细胞的保护作用。方法:以高糖(25 mmol/L)培养鼠肾小球系膜细胞。试验随机均分为正常对照(正常培养基)组、溶剂对照(0.1%DMSO)组、模型(正常培养基)组、卡托普利(1μmol/L)组与芦丁高、中、低质量浓度(40、10、5μg/ml)组,培养48 h。采用实时荧光定量-聚合酶链反应(RT-PCR)法测定细胞转化生长因子(TGF)-β1mRNA的表达,Western blot法检测细胞Smad 2/3与Smad 7的含量,分光光度法测细胞总超氧化物歧化酶(T-SOD)、丙二醛(MDA)、过氧化氢酶(CAT)与谷胱甘肽过氧化酶(GSH-Px)水平。结果:与溶剂对照组比较,模型组细胞Smad 2/3、TGF-β1mRNA的表达增强,T-SOD、CAT与GSH-Px活性减弱,MDA含量增加,Smad 7的表达减弱,差异有统计学意义(P<0.01)。与模型组比较,芦丁高、中、低质量浓度组细胞Smad 2/3表达减弱,T-SOD、CAT活性增强,MDA含量减少,Smad 7的表达减弱;芦丁高、中质量浓度组TGF-β1mRNA表达减弱,GSH-Px活性增强,差异有统计学意义(P<0.01或P<0.05)。结论:芦丁通过TGF-β1/Smad通路对高糖培养的大鼠肾小球系膜细胞产生保护作用,具有潜在的防治糖尿病肾病作用。更多还原

【Abstract】 OBJECTIVE:To study the protective effect of rutin on mesangial cells of high glucose model rats. METHODS:The mesangial cells of rats were cultured with high glucose. The cultured mesangial cells were randomly divided into normal control group(normal culture medium),solvent control group(0.1% DMSO),model group(normal culture medium),captopril group(1 μmol/L)and rutin high-concentration,medium-concentration and low-concentration groups(40,10,5 μg/ml),for culturing 48 h. The mRNA expression of TGF-β1in mesangial cells was detected by RT-PCR;the contents of Smad2/3 and Smad 7 were determined by Western blot;the levels of T-SOD,MDA,CAT,and GSH-Px were measured by spectrophotometry. RESULTS:Compared with solvent control group,the mRNA expression of Smad 2/3 and TGF-β1and MDA content were increased in model group,while the activities of T-SOD,CAT and GSH-Px and the expression of Smad 7 were decreased;there was statistical significance(P<0.01). Compared with model group,the expression of Smad 2/3 Smad 7,and MDA content were decreased in rutin high-concentration,medium-concentration and low-concentration groups,while the activities of T-SOD,CAT were increased;the mRNA expression of TGF-β1mRNA were decreased,GSH-Px was increased in rutin high-concentration,medium-concentration groups,there was statistical significance(P<0.01,P<0.05). CONCLUSIONS:Rutin can protect mesangial cells of high glucose model rats via TGF-β1/Smad pathway,and is a potent protective agent against diabetic nephropathy.更多还原