【摘要】 目的探讨辛伐他汀对糖基化终末产物(AGEs)诱导内皮细胞环氧化酶2(COX-2)表达的影响。方法体外以不同浓度的糖基化白蛋白(AGE-BSA)、辛伐他汀单独或联合作用于人脐静脉内皮细胞,检测细胞COX-2mRNA的表达及培养上清液中前列腺素E2(PGE2)的含量。结果 AGEBSA诱导内皮细胞COX-2mRNA的表达,作用呈剂量和时间依赖方式。辛伐他汀可降低AGE-BSA诱导的COX-2mRNA表达及PGE2产物浓度。结论 AGEs促进内皮细胞COX-2表达,辛伐他汀可减轻此作用。更多还原

【Abstract】 Objective To investigate the effect of simvastatin on advanced glycation end products(AGEs)induced cyclooxygenase-2(COX-2)mRNA expression in human umbilical vein endothelial cells(ECV304).Methods ECV304 were incubated with different concentrations of AGE-BSA and simvastatin singlely and combinedly for different hours.And the mRNA expression of COX-2and the supernatant content of PGE2 were tested.Results After treatment with AGE-BSA concentrations of 50,100,200and 400 mg/L,the mRNA expressions of COX-2 were increased in a dose-dependent manner(P<0.05).After treatment with 200 mg/L AGE-BSA for 6,12,24or 48h,the mRNA expression of COX-2were increased in a time-dependent manner(P<0.05).Incubation with simvastatin at 1,10and 100 μmol/L as add-on treatment to 200mg/L AGE-BS markedly prevented the up-regulation of COX-2mRNA expression,which correlated with an decrease in PGE2 level(P<0.05).Conclusion AGEs induce the expression of COX-2and PGE2 of endothelial cells in a dose-and time-dependent manner,these effects could be inhibited by simvastatin.更多还原