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响应面法优化杨梅渣中花色苷提取工艺

Optimization of Extraction Technique of Anthocyanins from Red Bayberry by Response Surface Methodology

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【作者】 刘青梅; 高娟; 姜敏芳; 杨性民; 孙金才;

【Author】 Liu Qingmei;Gao Juan;Jiang Minfang;Yang Xingmin;Sun Jincai;Ningbo Key Laboratory of Agricultural Products Processing Technology, Zhejiang Wanli University;Haitong Food Group Company;

【机构】 浙江万里学院宁波市农产品加工实验室; 海通食品股份有限公司;

【摘要】 以杨梅渣为原料,在单因素试验基础上,采用响应面法研究超声波辅助提取对杨梅渣中花色苷提取率的影响。分析结果表明:回归方程失拟项不显著(P=0.5225),预测值与实际值非常吻合(R2=0.9442)。试验因素对杨梅渣花色苷提取的影响依次为超声功率、料液比,提取时间。多因素方差分析结果显示,超声功率对花色苷提取率的影响极为显著(P<0.01),料液比对花色苷提取率的影响显著(P<0.05),而提取时间对花色苷提取率的影响不显著(P>0.05);超声功率、料液比与提取时间对花色苷提取率的交互作用不显著(P>0.05)。最佳提取工艺为:超声功率350 W、料液比7∶1、提取时间40 min,在此条件下提取花色苷含量为20.29 mg/100 g。

【Abstract】 Red bayberry was selected as the raw material. On the basis of single-factors experiments, the response surface methodology was used to study the extraction efficiency of anthocyanin with the aid of ultrasonic energy. The results of regression analysis showed a high coefficient of determination value(R2) of 0.9442 for the response surface model, which suggests a satisfactory agreement of the polynomial regression equation with experimental data. The influence of the conditions on extraction follows the sequence: ultrasonic power, ratio of material to liquid, extraction time. The analysis of variance indicated that ultrasonic power had an extremely significant effect on the extraction rate(P<0.01), ratio of material to liquid had a significant effect(P<0.05), extraction time had no significant effect(P>0.05), The interplay of these factors were not significant. the optimum extractive conditions were: ultrasonic power 350 W, ratio of material to liquid 7∶1, extraction time 40 min. under this condition the extraction of anthocyanin rate was 89.23%.

【基金】 浙江省科技厅公益性项目(2012C22094)
  • 【文献出处】 中国食品学报 ,Journal of Chinese Institute of Food Science and Technology , 编辑部邮箱 ,2014年06期
  • 【分类号】TS264.4;TS209
  • 【被引频次】25
  • 【下载频次】457
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