【摘要】 目的研究可乐定对永久性脑梗死大鼠的神经保护作用和对神经再生相关因子含量及神经干细胞增生的影响。方法成年雄性Sprague-Dawley大鼠,随机分为假手术组、缺血对照组、可乐定治疗组,制作大鼠永久性大脑中动脉栓塞模型,治疗组于术后3 h腹腔注射可乐定50μg/kg、100μg/kg(每天一次,连续5 d,或至实验终点);缺血对照组给予等剂量的生理盐水,通过神经行为学评分、尼氏染色、脑梗死体积测定、ELISA、BrdU/nestin免疫荧光双标等方法,分别观察可乐定治疗对大鼠神经损伤、功能恢复、神经再生相关因子表达水平、脑内相关部位神经干细胞增生的影响。结果与缺血对照组相比,可乐定治疗能改善大鼠神经行为学评分、减少损伤侧脑组织神经细胞丢失、缩小脑梗死体积、使得增生的神经干细胞数目及神经再生相关因子的含量进一步增加。结论可乐定对永久性脑梗死大鼠脑组织具有神经保护作用,且能促进神经再生相关因子在脑组织中的表达、诱导神经干细胞的增生,从而促进大鼠缺血脑损伤后的神经功能恢复。更多还原

【Abstract】 Objective The aim of this study was to investigate the neuroprotective effect of clonidine and its influences on the proliferation of neural stem cells and the expression of neuronal regeneration-related factors in brain tissue of rats after permanent middle cerebral artery occlusion. Methods Adult male sprague-dawley rats were subjected to permanent middle cerebral artery occlusion( PMCAO) and randomly divided into sham-operated,vehicle and clonidine-treated groups. Clonidine was intraperitoneally injected for consecutive 5 days or to end point of the experiment at a dose of 50 and100 μg /kg /day,with first use being started 3 h after PMCAO. The vehicle group was injected with isodose of normal saline.Neurobehavioral test,Nissl staining,TTC staining,ELISA and BrdU /nestin immunofluorescent double staining were conducted to determine the influence of clonidine treatment on the neurological function,neuronal loss of the injured side,infarct volume,expression of neuroregeneration-related factors,and proliferation of neural stem cells( NSCs) in the brain tissue.Results Compared with the vehicle group,treatment of clonidine greatly improved the neurological function of rats,reduced the neuronal loss and infarct volume,elevated the content of neuroregeneration-related factors,and increased the number of BrdU /nestin-positive cells in the brain tissue after PMCAO. Conclusion The present results suggest that clonidine could exert a neuroprotective effect,and promote the expression of neuroregeneration-related factors and proliferation of the endogenous NSCs in the brain tissue,thus contributing to better recovery of rats after PMCAO.更多还原