【摘要】 目的研究姜黄素及其单羰基结构衍生物B06对不同辐射抗拒鼻咽癌细胞株CNE-2与CNE-2R的作用。方法以MTT法检测姜黄素和B06对细胞增殖的影响,以流式细胞仪检测姜黄素和B06对细胞凋亡的影响,以Western-blot检测姜黄素和B06对ERS通路相关蛋白的影响。结果姜黄素对CNE-2和CNE-2R的IC50分别为8.35μM和6.84μM,B06对CNE-2和CNE-2R的IC50分别为1.09μM和0.74μM。B06能激活CNE-2R细胞ERS通路中关键蛋白CHOP、XBP-1、ATF-4的表达,而姜黄素则不能激活其表达。结论新型单羰基姜黄素衍生物B06能更显著地抑制辐射抗拒鼻咽癌细胞的增殖,该作用机制很可能是通过ERS通路发挥,提示B06具有抑制辐射抗拒鼻咽癌细胞的潜在价值。更多还原

【Abstract】 Objective To study the effects of B06, a mono-carbonyl derivative of curcumin, on homologous nasopharyngeal carcinoma cell lines CNE-2R and CNE-2 with different radioresistance. Methods The effects of B06 and curcumin on cell proliferation were determined by MTT assay. The cell apoptosis was determined by flow cytometry. The effects of B06 and curcumin on ERS related proteins were determined by western-blot. Results Curcumin inhibited CNE-2 and CNE-2R cells with IC50 of 8.35 μM and 6.84 μM respectively. B06 inhibited CNE-2 and CNE-2R cell lines with IC50 of 1.09 μM and 0.74 μM respectively. B06 could activate the expression of CHOP, XBP-1 and ATF-4, the key proteins of ERS passway, while curcumin could not. Conclusion The new mono-carbonyl curcumin derivative B06 can suppress the proliferation of nasopharyngeal carcinoma cells with radioresistance more significantly. The mechanism of this effect is probably through the ERS pathway, suggesting that B06 has the potential value to inhibit nasopharyngeal carcinoma cells with radioresistance.更多还原