【摘要】 目的研究冠心宁注射液的化学成分。方法利用反复硅胶柱色谱、ODS柱色谱、Sephadex LH-20柱色谱、大孔吸附树脂柱色谱、制备高效液相色谱和重结晶等方法进行分离纯化,并通过分离得到的各个化合物的理化性质和光谱数据鉴定化合物结构。结果分离得到19个化合物,分别鉴定为原儿茶醛(1)、香草醛(2)、阿魏酸(3)、2-糠酸(4)、洋川芎内酯I(5)、咖啡酸(6)、丹酚酸A(7)、异丹酚酸A(8)、丹酚酸B(9)、异丹酚酸C(10)、迷迭香酸(11)、1,3-二咖啡酰奎宁酸(12)、洋川芎内酯H(13)、对羟基苯甲酸(14)、间羟基苯甲酸(15)、邻羟基苯甲酸(16)、对羟基肉桂酸(17)、迷迭香酸甲酯(18)和丹酚酸N甲酯(19)。结论 19个化合物均为首次从该制剂中分离得到;经HPLC分析检测及文献对照,化合物2、3、5、13~16为川芎中的化学成分,化合物7~11、18~19为丹参中的化学成分,化合物1、4、6、12、17为川芎和丹参共有成分。更多还原

【Abstract】 Objective To investigate the chemical constituents from Guanxinning Injection.Methods The chemical constituents were repeatedly isolated by column chromatographic techniques including silica gel,ODS,Sephadex LH-20,and macroporous resin,and were purified by preparation HPLC chromatography and recrystallization,and their structures were identified by physicochemical properties and spectroscopic data.Results Nineteen compounds were isolated and identified as protocatechuic aldehyde(1),vanillin(2),ferulic acid(3),2-furoic acid(4),senkyunolide I(5),caffeic acid(6),salvianolic acid A(7),isosalvianolic acid A(8),salvianolic acid B(9),isosalvianolic acid C(10),rosmarinic acid(11),1,3-dicaffeoylquinic acid(12),senkyunolide H(13),4-hydroxybenzoic acid(14),m-hydroxybenzoic acid(15),o-hydroxybenzoic acid(16),4-hydroxycinnamic acid(17),methyl rosmarinate(18),and salvianolic acid N methyl ester(19).Conclusion Nineteen compounds are isolated from the Guanxinning Injection for the first time,and all HPLC analyses and literature data show that compounds 2,3,5,13—16 are originated from Ligusticum chuanxiong,compounds 7—11,and 18—19 from Salvia miltiorrhiza,and compounds 1,4,6,12,and 17 from L.chuanxiong and S.miltiorrhiza.更多还原