【摘要】 目的鉴定白头翁皂苷D在大鼠离体肠道菌群体外代谢产物。方法运用超高效液相色谱-三重四级杆线性离子肼复合质谱(UPLC-Q-trap-MS)分析白头翁皂苷D体外肠道菌群孵育样品,采用Analyst 1.5软件进行数据处理,根据保留时间和各色谱峰质谱裂解规律,比较确定白头翁皂苷D在体外肠道菌群中代谢产物,同时根据质谱二级裂解规律对代谢产物结构进行推测。结果在大鼠离体肠道菌群孵育样品中鉴定了白头翁皂苷D(M0)原型及常春藤皂苷元3-O-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖苷(M1)、常春藤皂苷元3-O-β-D-吡喃葡萄糖-(1→4)-α-L-吡喃阿拉伯糖苷(M2)、常春藤皂苷元(M3)、白头翁皂苷D羟基化(M4)、白头翁皂苷D甲基化产物(M5、M6)、白头翁皂苷D脱氢产物(M7)等7个代谢产物。结论白头翁皂苷D能够在大鼠离体肠道菌群中发生广泛的代谢。更多还原

【Abstract】 Objective To identify the metabolites of Pulsatilla saponin D(PSD) in the intestinal microflora of rats in vitro.Methods The sample of PSD incubated in intestinal microflora of rats in vitro was analyzed by a ultraperformance liquid chromatography/hybrid triple quadrupole linear ion trap mass spectrometry(UPLC-Q-trap-MS).The data were processed with Analyst 1.5 software,and the metabolites of PSD were confirmed by comparing the retention time and MS/MS spectra.The possible structures of metabolites were proposed by analyzing the proposed fragmentation pathway.Results PSD(M0,parent) and seven metabolites were identified,including hederagenin 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinosyl(M1),hederagenin 3-O-α-L-glucopyranosyl-(1→4)-α-L-arabinosyl(M2),hederagenin(M3),hydroxylation of PSD(M4),methylation of PSD(M5 and M6),and dehydrogenation of PSD(M7).Conclusion PSD has a wide range of metabolic in the intestinal microflora of rats in vitro.更多还原