【摘要】 目的检测去唾液酸糖蛋白受体2(asialoglycoprotein receptor 2,ASGR2)基因在肝细胞性肝癌(hepatocellular carcinoma,HCC)中的表达水平,探讨ASGR2在HCC发生、发展中的作用和意义。方法以RT-PCR法检测55例HCC患者、55例非肝癌的其他肿瘤患者和33例非肿瘤的其他疾病对照患者外周血液中ASGR2基因的表达水平,并分析其意义。结果肝癌组和非肝癌的其他肿瘤组患者血液中ASGR2基因阳性表达率分别为85.5%、65.5%,两组差异有统计学意义(P<0.05),肝癌组和非肿瘤的其他疾病对照组ASGR2基因阳性表达率分别为85.5%、78.8%,两组差异无统计学意义(P>0.05),ASGR2基因在3组患者外周血液中的表达水平有明显差异(P<0.05)。ASGR2基因的表达与HCC患者的性别、年龄、AFP、肝硬化、肿瘤家族史等临床病理特征均无统计学意义(P>0.05)。结论 ASGR2基因的表达水平可能与HCC发生、发展有关,检测ASGR2的水平有望作为HCC早期发病的预测指标。更多还原

【Abstract】 Objective To explore the expression and possible significance of asialoglycoprotein receptor 2(ASGR2)gene in hepatocellular carcinoma(HCC). Methods Expression of the ASGR2 gene in peripheral blood was detected by RT-PCR in 55 patients with hepatocellular carcinoma,55 patients with non-HCC tumors,and 33 non-tumor control patients. Results Expression of the ASGR2 gene was detected in significantly more HCC patients(85.5%)than in non-HCC patients(65.5%,P<0.05);in fact,the rate of positive expression was similar among HCC patients as in non-cancer controls(78.8%,P>0.05).Gene expression differed significantly across all three patient groups(P<0.05).ASGR2 gene expression was not significantly associated with clinicopathological features of HCC,age, AFP,cirrhosis,or cancer family history(P>0.05). Conclusion ASGR2 gene expression may be associated with HCC development, and detecting expression may serve as an early predictor of pathogenesis.更多还原