【摘要】 胃癌是一种由基因和环境因素相互作用、在世界范围内最常见的恶性肿瘤之一.该研究用PCRRFLP的方法,在270名健康对照个体和239名胃癌患者共509人的云南汉族人群中,对核酸外切酶1(exonuclease 1,EXO1)基因rs1635517、rs1776177、rs851797、rs1635498和rs1776148五个单核苷酸多态(single nucleotide polymorphisms,SNPs)位点进行多态检测.结果采用SPSS16.0、PHASE、HaploView4.2软件进行数据统计分析,用Logistic模型在95%置信区间内进行相关性风险度测算.结果显示,位于EXO1基因5’UTR的位点rs1635517的CC基因型携带者和C等位基因携带者患胃癌的相对风险度显著升高(p<0.05),提示该多态位点可能与胃癌发生相关.其余4个位点虽检测到多态,但它们的基因型和等位基因频率在病例和对照中无显著差异,提示这4个位点可能与云南汉族人群的胃癌发生不相关.更多还原

【Abstract】 Gastric cancer(GC) is one of the most common malignant cancers,which is influenced by both genetic and environmental factors.The relevance between five SNPs(rs1635517,rs1776177,rs851797,rs1635498,rs1776148) of EXO1 gene and gastric cancer(GC) was investigated in 509 individuals(270 healthy individuals and 239 patients with GC) from Yunnan Han Population.Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The statistical software package SPSS16.0,PHASE and HaploView4.2 was used.The results showed that both C allele and CC genotype of rs1635517 which located in the 5’ UTR of EXO1 gene might be the risky factor of GC(adjusted p < 0.05),while the other 4 SNPs of EXO1 genes did not appear to influence the susceptibility to GC.更多还原