【摘要】 以弗氏链霉菌CK6-6为出发菌株,分别采用紫外(UV)、甲基磺酸乙酯(EMS)、紫外与氯化锂(LiCl)复合、甲基磺酸乙酯与紫外复合4种方法对其进行诱变。比较了4种方法的最大提高率、致死率、正变率及突变株的传代稳定性,结果发现诱变效果较好的为EMS与UV复合诱变,筛选出1株传代稳定菌株EU45-15,相对于出发菌株CK6-6,泰乐菌素产量的提高率为15.36%。经HPLC分析发现,它的最高活性组分A的含量高于对照,提高率为48.28%。同时利用豆油作为筛选物质对弗氏链霉菌进行定向筛选,筛选效果较好,筛出1株高产稳定菌株O3.6-8,效价提高率为12.15%。更多还原

【Abstract】 Ultraviolet(UV),ethyl methane sulphonate(EMS),UV and lithium chloride(LiCl) compound,EMS and UV compound were used for mutation screening on CK6-6. By comparing the maximum increase rate,the mortality rate,positive rate and the stability of mutants,we found that EMS and UV compound irradiations displayed better mutation effect on Streptomyces fradiae CK6-6. By shake flask screening,one stable high-yielding mutants was obtained: EU45-15. Tylosin yields was increased by 15. 36%. HPLC analysis showed that the percentage of the highest active component A of EU45-15 was 48. 28%,higher than that of control. Simultaneously,soybean oil was used to screen high-yielding strain of S. fradiae and proved to have good effect. In the selected mutant O3. 6-8,the production of tylosin was increased by 12. 15%.更多还原