【摘要】 利用响应面法优化天然蜂蜡的皂化水解工艺,并采用红外光谱法和气相色谱法对水解产物之一的棕榈酸进行定性及定量分析。分别对天然蜂蜡皂化反应的温度、时间、氢氧化钠浓度、液固比等条件进行响应曲面试验设计,建立天然蜂蜡皂化水解的最佳工艺。通过气相色谱法对天然蜂蜡皂化水解并经酸化处理后得到的脂肪酸中棕榈酸的含量进行分析测定。结果表明,最佳皂化水解工艺为氢氧化钠乙醇溶液1.89 mol/L,皂化时间4.5 h,皂化温度87℃,液固比10.05︰1(mL/g)。在此试验条件下,天然蜂蜡的皂化率可达97.49%,天然蜂蜡脂肪酸中棕榈酸的含量为61.32%。更多还原

【Abstract】 The saponification technology of natural beeswax was optimized, infrared spectra and gas chromatographic method were applied to determine the quality and quantity of the palmitic acid. The saponification conditions of natural beeswax, such as temperature, time, sodium hydroxide concentration, liquid to solid ratio, were analyzed by response surface method. An optimum process of saponification was identified with the sodium hydroxide concentration 1.89 mol/L, the saponification time 4.5 h, the saponification temperature 87 ℃, the liquid to solid ratio 10.05︰1(mL/g). Under the best condition, saponification rate was 97.49% with 61.32% palmitic acid of fatty acid in nature beeswax.更多还原