【摘要】 为了探索丙二醛对小鼠骨髓间充质干细胞(MSCs)凋亡的诱导作用及其机制,在不同浓度的丙二醛培养体系中孵育MSCs 24 h,用TUNEL法、流式细胞术检测MSC凋亡率,并用实时定量RT-PCR、Western印迹检测Bcl-2、Bax及Caspase-3基因的表达水平。结果发现,MDA能浓度依赖性地增加TUNEL阳性细胞百分率、亚G1峰细胞百分率,同时下调Bcl-2 mRNA及蛋白的表达,上调Bax mRNA和Caspase-3 mRNA及蛋白的表达.这些结果表明:在体外培养条件下,丙二醛可诱导小鼠骨髓间充质干细胞的凋亡,其作用机制与Bcl-2、Bax和Caspase-3基因表达水平的变化有关。更多还原

【Abstract】 In order to explore the apoptosis-inducing effect of MDA on murine bone marrow-derived mesenchymal stem cells(MSCs) in vitro, MSCs were treated with and without three concentrations of MDA(0.01, 0.1, 1.0 mmol/L) for 24 h.The apoptotic indexes were evaluated by flow cytometry and TUNEL assay. mRNAs and proteins levels of Bcl-2, Bax and Caspase-3 were measured using Q-RT-PCR and Western blotting. MDA caused apoptosis in MSCs,increased the expression of Bax and Caspase-3, and decreased the expression of Bcl-2 in a concentration-dependent manner. MDA-induced apoptosis is involved in the change in expression levels of Bcl-2, Bax and Caspase-3.更多还原