【摘要】 目的制备六臂星形聚己内酯并评价其生物相容性。方法采用本体开环聚合法以ε-己内酯为单体,辛酸亚锡为催化剂,肌醇为多功能基团引发剂,合成一系列不同相对分子质量的六臂星形聚己内酯(6-s-PCLx)(其中x为单体与引发剂的摩尔比)。通过傅里叶红外光谱(FT-IR)、核磁共振氢谱(1H-NMR)、凝胶渗透色谱(GPC)、差示扫描热分析法(DSC)对其结构、相对分子质量及热力学性质进行表征。以此材料为载体,采用超声乳化/溶剂挥发法,制备空白纳米粒。采用动态光散射法(DLS)和扫描电子显微镜(SEM)对其粒径和形态进行表征,通过WST-1比色法探讨该材料的体外细胞毒性。结果 FT-IR和1H-NMR表明,该聚合物为六臂星形结构;由GPC得出其相对分子质量分别为22 822、31 459、49 533,并呈单峰分布;DSC结果表明,聚合物的熔融温度(Tm)、熔融焓(ΔH)及结晶度(Xc)随着相对分子质量的增加而升高;制备的纳米粒呈椭圆形,平均粒径在100~200 nm,粒径均一;WST-1比色法证明,该材料具有良好的生物相容性。结论成功合成六臂星形聚己内酯并制备了纳米粒,细胞实验证明该材料具有良好的生物相容性。更多还原

【Abstract】 Objective To prepare a series of six-arm star-shaped poly(ε-caprolactone)(6-s-PCL) with different molecular weight and evaluate their biocompatibility. Methods The polymers were synthesized by stannous octoate catalyzed ring-opening polymerization of ε-caprolactone with inositol as multifunctional initiator. The structure of the polymers was characterized with Fourier transform infrared spectrometer(FT-IR) and nuclear magnetic resonance spectroscope(1H-NMR), the molecular weight and polydispersity index were tested by gel exclusion chromatography(GPC). The thermal behavior of polymers was analyzed by differential scanning calorimetry(DSC). Subsequently, the nanoparticles of 6-s-PCL were prepared by ultrasound/solvent evaporation method, and dynamic light scattering(DLS) was adopted to investigate the size and potential of nanoparticles. The cytotoxicity of 6-s-PCL was detected by water soluble tetrazolium salt reagent(WST-1) assay. Results The nanoparticles prepared from 6-s-PCL exhibited irregular spherical shape as shown by FT-IR and1H-NMR, and the molecular weight determined by GPC was 22 822, 31 459, and 49 533, respectively, and distributed unimodally. The result of DSC showed that the melting temperature(Tm), melting enthalpy(ΔH) and crystallinity(Xc) were increased with increasing molecular weight. The nanoparticles were in uniform oval shape, with mean diameter of 100- 200 nm. In addition, the polymers showed excellent biocompatibility as demonstrated by WST-1 assay. Conclusion The 6-s-PCL is prepared successfully, which demonstrates good biocompatibility and might be an appropriate candidate as drug carriers.更多还原