【摘要】 目的研究水通道蛋白AQPs在双峰驼肺中的表达情况,探讨双峰驼适应极干旱荒漠环境的呼吸生理机制。方法运用常规形态学统计方法和石蜡切片HE染色法对双峰驼肺组织形态结构进行统计与分析,免疫组化方法对双峰驼肺中AQPs的表达进行定位分析。结果双峰驼气管长且弯曲,肺较致密且含水量较黄牛高。免疫组化检测显示,在双峰驼肺中有AQP1、AQP3、AQP4和AQP5四种AQPs表达。其中,AQP1主要表达于肺毛细血管网、淋巴管以及气管上皮细胞顶膜面;AQP3主要表达于气管上皮基底细胞质膜上;AQP4主要分布于整个气管上皮杯状细胞基底侧细胞膜和肺泡Ⅱ型上皮细胞;AQP5表达于气管粘膜下腺腺体细胞管腔面和肺泡Ⅰ型上皮细胞膜上。结论呼吸道和肺组织形态学特征表明双峰驼对干旱沙漠环境具有很好的适应性,AQPs在双峰驼肺中的强烈表达,与气道润化、气道水平衡、气道表面液体层、肺内液体转运和肺内水平衡等生理过程有关,为其适应极干旱荒漠环境提供了分子生物学依据。更多还原

【Abstract】 Objective In order to provide a molecular basis for the adaptation mechanism of Bactrian camel( Camelus bactrianus) to the extremely arid environment,the respiratory morphological characteristics of bactrian camel lung and the expression of aquaporins were determined in this study. Methods Paraffin sections and statistical methods were used to study the structure and ultrastructure morphology. And the immunohistochemical methods were used to analyze the expression and localization of aquaporins in the lung. Results Bactrian camel had a long and curved tracheal,and the lung was more compact and had higher water than cattle. Immunohistochemical detection results showed that there were four aquaporins,including AQP1,AQP3,AQP4 and AQP5 in the lung of the bactrian camel. AQP1 was mainly expressed in the capillary network of respiratory tract and tracheal epithelial cells in the apical membrane surface. AQP3 was present on the surface of respiratory epithelial and the basement membranes of gland cells. AQP4 was distributed throughout the basolateral membranes of the airway epithelial goblet cells and the alveolar epithelial cells Ⅱ. AQP5 was distributed on the luminal surface of the gland glandular cells and the alveolar epithelial cellsⅠ. Strong expression of aquaporins in the lung of the bactrian camel were related to the physiological function,such as airway humidification and water balance,airway surface liquid regulation and control,lung fluid transportation and water balance in lung et al.,which provided a molecular biological explanation.更多还原