【摘要】 目的了解2011-2012年深圳地区致病性呼吸道腺病毒(AdV)的病原体基因亚型和遗传进化及其流行特征,分析深圳地区呼吸道腺病毒的流行规律和传播途径。方法收集2011年1月至2012年12月深圳地区31个流感样病例监测哨点2 822份样本,先用荧光定量PCR方法筛查流感样病例,筛查后的阴性样本进一步进行呼吸道腺病毒等13种常见呼吸道病毒的核酸检测。对检测出的109份腺病毒阳性标本进行病毒分离,感染Hep-2受体细胞后共获得71份AdV稳定细胞毒株,用腺病毒六邻体基因作为靶基因,扩增其中的50份腺病毒阳性标本,纯化扩增产物后直接用于序列分析,在GenBank上进行序列比较,确定其病毒亚型并进行系统进化分析。结果 2011-2012年深圳市腺病毒感染患者的高发年龄段人群为小于7岁的儿童,1~2岁婴幼儿发病率最高;4-6月和10-12月为每年发病高峰期。在2 822份流感样病例咽拭子标本中,经定量PCR方法确定为AdV核酸阳性共109份,核酸阳性率为3.86%,从109份核酸阳性样本中分离病毒71份,病毒分离率为68.93%。50份分离的呼吸道腺病毒分子分型结果为1型AdV 6例,占12%;2型AdV 4例,占8%;3型AdV 27例,占54%;4型AdV 3例,占6%;5型AdV 4例,占8%;7型AdV 5例,占10%;另外有复合型AdV(HAdV-un)1例,占2%。结论近两年来深圳地区呼吸道Adv感染以3型为主,腺病毒引起的呼吸道疾病主要发生在每年的4-6月,感染人群主要是1~2岁婴幼儿。更多还原

【Abstract】 Objective To identify the genotypes,genetics, evolution and spreading characteristics of respiratory adenovirus(AdV) in Shenzhen. Methods 2 822 nasal and throat swabs samples from 31 sentinel hospitals in Shenzhen were collected. Influenza virus was detected using real-time PCR. Negative samples were further tested for 13 various types of respiratory AdV. 109 AdV positive samples were confirmed by PCR.Using the Hep-2 cell culture method,71 AdV isolates were obtained from 109 AdV-positive samples.50 virus isolates were further analyzed and the hexon gene of AdV was sequenced. The sequences were compared with the Genebank data. Results According to the epidemiological study, children aged less than 7 years old were the most susceptible population group,and the highest morbidity group was the children of 1-2 years old. High incidence of the occurrence was observed from April to June and from October to December. Among the 2,822 nasal / throat swabs samples, 109(3.8%) samples were found to be AdV positive by the PCR method,and 71 virus isolates(68.93%) were obtained. In the AdV subtype analysis(50 ADV samples),the results were as following : 6(12%) isolates were HAdV-1,4(8%) isolates were HAdV-2,27(54%) isolates were HAdV-3,3(6%) isolates were HAdV-4, 4(8%) isolates were HAdV-5, 5(10%) isolates were HAdV-7 and 1(2%) was coinfection(HAdV-un). Conclusions AdV-3 was the most frequently detected serotype of respiratory AdV infection in Shenzhen. Respiratory disease caused by AdV occurs mainly during April to June and in infants aged 1 to 2.更多还原