【摘要】 目的建立对四种鼠药的实验室应急检测方法,以应对突发公共卫生毒物中毒事件。方法采用气质联用法(GC/MS)测定毒鼠强、氟乙酰氨和高效液相色谱法(HPLC)测定敌鼠钠、溴敌隆,以鼠药标准和鼠药样品为对象,优化GC/MS和HPLC色谱、质谱条件,从而建立各鼠药的色谱图库和质谱图库。结果 GC/MS定性检测毒鼠强的特征离子和丰度比分别为212/240和2:1,氟乙酰胺的分别为44/77和1.5:1;敌鼠钠、溴敌隆以高效液相色谱双波长(285nm和260nm)检测,固体样品最低检测浓度为0.80 mg/kg,液体样品最低检测浓度为0.16 mg/L。结论研究方法对中毒事件中四种鼠药检测快速、结果可靠,适用于鼠药中毒实验室应急检测。更多还原

【Abstract】 Objective To establish laboratory emergence detection of four rat posisons for sudden public healthpoisoning events. Method Tetramine, fluoroacetamide were detected by GC/MS, and diphacin and bromadiolone by HPLC.The GC/MS,HPLC chromatography and mass spectrometry conditions was optimized to establish chromatographic gallery andmass pectrometry gallery of each rat poison. Result The characteristic ions and abundance ratio of GC/MS qualitativedetection for tetramine were 212/240 and 2:1, respectively, and those for fluoroacetamide were 44/77 and 1.5:1; respectively.The minimum detectable concentrations of solid samples and liquid samples detected by HPLC using dual-wavelength(285nmand 260nm)in the diphacin and bromadiolone were 0.80mg/kg and 0.16ug/L, respectively. Conclusion The method israpid, accurate and suitable for rapid detection of rodenticide poisoning.更多还原