【摘要】 采用包被抗体、酶标半抗原直接竞争酶联免疫吸附分析法(ELISA)测定了氰戊菊酯在桃中的残留量。结果表明:在氰戊菊酯多克隆抗体包被浓度为4.0 mg/L、辣根过氧化物酶(HRP)标记氰戊菊酯半抗原稀释1.0×105倍条件下,ELISA法检测氰戊菊酯的线性浓度范围为0.01~10 mg/L,氰戊菊酯对抗体-酶标半抗原反应的抑制中浓度(IC50)为193μg/L,相对标准偏差(RSD,n=5)为4.5%,IC20为13.5μg/L。在2、0.2和0.05 mg/kg添加水平下,ELISA法测定桃中氰戊菊酯的回收率分别为81%~89%、85%~98%和85%~106%,RSD(n=5)分别为5.1%、5.6%和7.7%。ELISA法对桃中氰戊菊酯的最低检出浓度为0.014 mg/kg。更多还原

【Abstract】 The method of antibody immobilized direct competitive enzyme-linked immunosorbent assay( ELISA) was employed to determine fenvalerate residue in peach. The result showed that the linear concentration of ELISA ranged from 0. 01 to 10 mg/L,the half maximal inhibitory concentration( IC50) of fenvalerate to antibody hapten-HRP reaction was 193 μg /L with relative standard deviation( RSD,n = 5) of 4. 5% and the IC20w as 13. 5 μg/L. The fenvalerate recoveries from peach determined by ELISA w ere 81%- 89%,85%- 98% and 85%- 106% w ith RSDs( n = 5) of 5. 1%, 5. 6% and 7. 7% at the spiked levels of 2 mg/kg,0. 2 mg/kg and 0. 05 mg/kg,respectively. The minimum detection concentration of the method w as 0. 014mg/kg.更多还原