【摘要】 目的:研究脂多糖诱导的炎症对雄性大鼠睾丸组织病理学和生殖内分泌功能的影响,初步探讨炎症影响雄性生育的可能机制。方法:36只雄性SD大鼠(400~450g)随机分为4组,每组9只。对照组(A组)大鼠腹膜内注射无菌生理盐水,余下3组大鼠腹膜内注射脂多糖(LPS,5 mg/kg,溶于无菌生理盐水中),分别于注药12 h(B组)、24 h(C组)和72 h(D组)后麻醉处死。取各组大鼠左侧睾丸,制成组织切片,HE染色,镜下观察睾丸组织病理学改变;取各组大鼠血清进行血清睾酮(T)、卵泡刺激素(FSH)和黄体生成素(LH)含量检测。结果:①大鼠睾丸组织HE染色显示:与A组相比,B组大鼠睾丸生精小管结构清晰,各级生精细胞排列整齐,部分生精小管管腔内精子数目有所减少,并可见脱落的生精细胞;C组大鼠睾丸生精上皮变薄,生精小管结构较紊乱、不规则,各级生精细胞减少且排列不整齐,生精小管管腔内成熟精子数目减少,并可见脱落的生精细胞;D组大鼠睾丸生精上皮变薄,生精小管结构紊乱、不规则,各级生精细胞明显减少且层次紊乱,生精小管管腔内成熟精子数目减少,可见较多生精细胞脱落并阻塞管腔。②各组大鼠血清T、LH、FSH含量,A组T为(0.490±0.028)ng/ml,LH为(6.290±0.515)ng/L,FSH为(1.837±0.127)IU/L;B组T为(0.460±0.024)ng/ml,LH为(5.881±0.124)ng/L,FSH为(1.707±0.098)IU/L;C组T为(0.417±0.021)ng/ml,LH为(5.123±0.271)ng/L,FSH为(1.620±0.115)IU/L;D组T为(0.378±0.021)ng/ml,LH为(4.504±0.279)ng/L,FSH为(1.562±0.216)IU/L;与A组相比,B组大鼠血清T、LH和FSH含量均降低,但无统计学差异(P>0.05);C、D组大鼠血清T和LH明显降低(P<0.01),FSH降低,有统计学差异(P<0.05)。结论:脂多糖(5 mg/kg)腹膜内注射诱导炎症可损害雄性大鼠睾丸组织,并影响大鼠生殖内分泌功能,导致血清T、LH和FSH含量降低,可能影响雄性生育。更多还原

【Abstract】 Objective: To study the influence of lipopolysaccharide( LPS)-induced inflammation on the testicular histology and reproductive endocrine function in male rats and investigate the possible mechanism of inflammation affecting male fertility. Methods: Thirty-six male SD rats were randomly divided into a control group( A) and three LPS intervention groups( B,C,and D) to receive saline and LPS( 5 mg / kg i. p,once),respectively. The animals in groups B,C,and D were killed by anesthesia at 12,24,and 72 hours after treatment. Histopathological changes in the left testis of the rats were observed by HE staining and the levels of the reproductive hormones T,FSH,and LH in the serum were determined by ELISA. Results: Compared with group B,group A showed clear structure of seminiferous tubules,orderly arrangement of spermatogenic cells,a slightly decreased number of sperm in some seminiferous tubular lumens,and shed spermatogenic cells in the rat testis tissue; group C exhibited thinner seminiferous epithelia,disordered structure of seminiferous tubules,irregular arrangement of spermatogenic cells,decreased number of mature sperm and obvious shedding of spermatogenic cells in seminiferous tubular lumens; group D manifested similar findings to those of group C,with even more shed spermatogenic cells that blocked the tubular lumens. The levels of serum T,LH,and FSH were( 0. 490 ± 0. 028) ng / ml,( 6. 290 ±0. 515) ng/L,and( 1. 837 ±0. 127) IU/L in group A,( 0. 460 ±0. 024) ng/ml,( 5. 881 ±0. 124) ng/L,and( 1. 707 ± 0. 098) IU / L in group B,( 0. 417 ± 0. 021) ng / ml,( 5. 123 ± 0. 271) ng / L,and( 1. 620 ± 0. 115) IU / L in group C,and( 0. 378 ± 0. 021) ng / ml,( 4. 504 ± 0. 279) ng / L and( 1. 562 ± 0. 216) IU / L in group D,all decreased in group B as compared with A( P > 0. 05). The decreases of T and LH were extremely significant( P < 0. 01) and that of FSH was significant in groups C and D( P < 0. 05) in comparison with A. Conclusion: LPS-induced inflammation affects the testicular tissue and reproductive endocrine function of male rats,resulting in decreased levels of serum T,LH,and FSH.更多还原