【摘要】 目的建立HPLC法测定哮喘颗粒中苦杏仁苷的含量。方法采用Agilent C18(250 mm×4.6 mm,5μm)色谱柱;流动相为甲醇-1%磷酸水(21∶79);流速:1.0 mL/min;检测波长:210 nm。结果苦杏仁苷的线性范围为0.161 4~3.228μg,r=0.999 8,平均回收率为99.02%,RSD=0.81%。结论该方法简便、准确,可作为该制剂的质量控制方法。更多还原

【Abstract】 Objective To establish an HPLC method for the determination of amygdalin in asthma granules.Methods The procedure of HPLC was performed on the chromatographic column of Agilent C18( 250 mm × 4. 6 mm,5 μm),the mobile phase consisted of methanol-1% phosphoric acid water( 21 ∶ 79). The flowrate was l m L / min and detection wave length was 210 nm. Results Amygdalin showed good linear relationship in the range of 0. 161 4 ~3. 228 μg,and the average recovery rate was 99. 02%,RSD = 0. 81%. Conclusion The established method is simple,accurate,and it can be used for quality control of asthma granules.更多还原