【摘要】 目的探讨神经纤维丝蛋白(NF)、突触素(SYN)在人胚胎脊髓发育阶段的分布规律及其表达意义。方法应用免疫组织化学法检测第2、3、4个月龄段,共16例人胚胎脊髓中NF、SYN的表达、分布状况。结果第2个月胚龄时,NF仅在人胚脊髓的前正中裂和后正中隔两侧的边缘层呈弱阳性表达。第3~4月龄时,NF在脊髓的前正中裂、后正中隔及皮质区域均呈阳性或强阳性表达,在室管膜层外侧的部分灰质内可见NF表达阳性的神经纤维。第2~4个月龄段,人胚胎脊髓内均可见SYN阳性表达,随着胎龄的增大,脊髓内SYN阳性表达强度值呈先升高再降低,阳性数量值呈逐月升高。结论 NF和SYN参与调控人胚胎脊髓组织的生长发育。更多还原

【Abstract】 Objective To investigate the neurofilament protein( NF) and synaptophysin( SYN) distribution in the developing stage of human embryonic spinal cord and their clinical significance. Methods Totally 16 of human embryos,aged from 2 to 4 months were used in this study. Immunohistochemical staining was uesd to detect the expression and distribution of NF and SYN in the spinal cord. Results At the second month stage,NF expression was weakly positive in only the marginal layer on both sides of the spinal cord. At 3-4 months,NF expression was positive or strong positive in the spinal cord center crack before and after the middle of partition and cortex. In the ependymal layer of the lateral part of the expression of NF positive nerve fibers were seen in the gray matter. From 2 to 4 month-age,all visible SYN positive expressions distributed in the spinal cord. With the increase of gestational age,SYN positive expression in the spinal cord staining intensity value was increased first,and then decreased. The number of positive values increased month by month.Conclusion NF and SYN both take part in the regulation of human embryonic spinal cord tissue growth.更多还原