【摘要】 目的探讨结肠癌组织中液泡型ATP合酶(V-ATPase)表达与结肠癌临床病理特征的关系及其对结肠癌发生发展的意义。方法随机选取20例结肠肿瘤组织和配对的正常组织,通过逆转录-聚合酶链反应(RT-PCR)和实时定量聚合酶链反应(Real-time PCR)检测V-ATPase基因的mRNA表达量,采用免疫组织化EnVinsion法检测V-ATPase基因在68例结肠癌组织及配对的正常癌旁组织中蛋白表达量。结果结肠癌组织及配对正常组织中V-ATPase基因mRNA平均表达量分别为(5.37±0.44)、(2.03±0.35)(P<0.01)。免疫组织化学结果显示V-ATPase在结肠肿瘤组织及配对正常组织中阳性表达率分别为69.1%(47/68)和5.8%(4/68),其阳性表达主要在胞膜和胞质中,V-ATPase过表达与肿瘤分期(P<0.05)、区域淋巴结转移(P=0.044)、远处转移(P=0.049)、脉管浸润(P=0.044)和分化(P<0.001)有关。结论 V-ATPase基因在结肠癌组织中高表达对结肠癌的发生发展起重要作用,可能为结肠癌术后辅助化疗的重要靶点。更多还原

【Abstract】 Objective To explore the expression of V-ATPase in colon cancer and its clinical significance. Methods Detecting the expression of V-ATPase mRNA in 20 paired of colon tumor tissues and normal tissues by using reverse transcription-polymerase chain reaction(RT-PCR)and real-time quantitative polymerase chain reaction(Real-time PCR),and testing the expression of V-ATPase protein by immunohistochemistry of EnVinsion. Results The expression of V-ATPase mRNA in tumor tissues and its paired normal tissues were(5. 37 ±0. 44) and(2. 03 ± 0. 35)(P < 0. 01). The positive immunohistochemistry of V-ATPase in tumor tissues and its paired normal tissues were69. 1%(47 /68) and 5. 8%(4 /68) respectively,and the positive expression were primarily in cytoplasm and cytomembrane. Overexpression of V-ATPase was associated with tumor stage(P < 0. 05),lymph node metastasis(P = 0. 044),distant metastasis(P = 0. 049),vessel invasion(P = 0. 044) and differentiation(P < 0. 001). Conclusion Overexpression of V-ATPase plays a significant role in the carcinogenesis and the progression of colon cancer,which might be an important postoperative therapeutic target.更多还原