【摘要】 为了筛选适宜的猪精液常温保存稀释液配方,在对稀释液pH、渗透压及缓冲指数进行理论控制的基础上,设计9个稀释液配方,以进口BTS稀释液为对照,通过分析精液有效保存时间和生存指数筛选出最佳配方,然后进行母猪输精试验。结果表明,稀释液pH、渗透压及缓冲指数进行理论控制方法的准确性分别达到97.1%~99.42%、98.7%~99.68%和98.48%~99.16%,可以满足精液稀释液配方设计的要求。9号极显著地高于5、6、7、8号稀释液(P<0.01),稀释液有效保存时间160.8h,生存指数49.46,显著高于8号液(P<0.05),极显著高于5、6、7号液(P<0.01)。9号液精液有效保存时间、生存指数、母猪情期受胎率和平均窝产仔数分别达到160.8h、49.46、80.5%和9.5头,与进口BTS液差异不显著(P>0.05),可用于实际生产。更多还原

【Abstract】 To screen a formulation of semen extender used in swine semen preservation in room temperature,nine extenders were designed with pH,osmotic pressure,and buffer index of semen extender under theoretical control,and then were analyzed in terms of effective preservation time and survival index in comparison to imported BTS extender so as to find a better one for sow artificial insemination.The result showed that the theoretical control accuracy of pH,osmotic pressure,and buffer index of semen extender were 97.1%~99.42%、98.7%~99.68% and 98.48~99.16%respectively,which were adequate for the design of semen extender.The 9th extender surpassed the rest with the sperm survial time being 160h,extremely significantly longer then the 5th,6th,7th and 8th(P<0.01),the survival index being 49.46,significantly higher than the 8th(P<0.05)and extremely significantly higher than the 5th,6th and 7th(P<0.01). The sperm survival time,index,sow pregnant ratio and litter size of 9th extender were 160.8h,49.46, 80.5%and 9.5respectively,there was no significant difference from the BTS extender(P>0.05),which means it can be used in the practice.更多还原