【摘要】 为了确定导致吉林省某地区10日龄雏鹅发病的病原,从病死雏鹅的肝脏中分离到一株病毒,根据病毒的电镜照片,动物回归试验及PCR鉴定,确定为鹅细小病毒。PCR产物测序后经BLAST比对显示,分离毒株与NCBI收录的GPV VP3基因同源性均在92%以上,与GPV/CH/HLJ02/08VP3基因的同源性最高,达99%。致病性研究表明,分离株的ELD50为10-6.5/0.2mL,毒力较强,且该毒株能被GPV标准血清所中和。更多还原

【Abstract】 A strain of Goose parvirus isolated from the livers of dead goslings in an area of Jilin province was identified to be the pathogen of the 10-day-old goslings disease based on its electron micrograph,animal regression test and PCR results.A comparison through BLAST showed that the VP3gene of the isolated strain had a homology of more than 92% with those of the GPV included in NCBI and a highest homology with those of GPV/CH/HLJ02/08,which was up to 99%.Pathogenicity research on it showed that its ELD50 is 10-6.5/0.2mL,a very strong virulence.And the strain can be neutralized by standard serum of the GPV.更多还原