【摘要】 目的分离并鉴定北京市输入性D9基因型麻疹毒株。方法使用Vero/SLAM细胞,对可疑麻疹输入性病例的咽拭子和尿液标本进行麻疹毒株的分离培养。用反转录-聚合酶链反应扩增麻疹病毒核蛋白(N)基因羧基末端676个核苷酸片段,对扩增产物进行核苷酸序列测定和分析,并以羧基末端450个核苷酸片段构建基因亲缘关系树,进行遗传距离及核苷酸同源性分析。结果该病毒分离株BJCY13026-2和世界卫生组织D9基因型代表株Victoria.AUS(维多利亚.澳大利亚)12.99在基因亲缘性关系树上同属一个分支,核苷酸同源性为95.8%,氨基酸同源性为96%;和其他23个基因型代表株的核苷酸和氨基酸同源性分别在88.1%~95.6%和90.7%~96.7%。和中国大陆目前所使用的麻疹疫苗株沪191相比对,其核苷酸和氨基酸同源性分别为91.1%和90.0%;和中国目前流行的麻疹病毒绝对优势本土基因型H1a基因型代表株相比对,其核苷酸和氨基酸同源性分别为89.4%和91.3%。结论该输入性病例的病毒分离株为麻疹病毒D9基因型。更多还原

【Abstract】 Objective To identify the genotype and molecular characteristic of imported measles virus genotype D₉.detected for the first time in Beijing.Methods The throat swabs and urine specimens were collected from the suspected imported measles patient to isolate virus by using Vero/SLAM cell line.Fragment of 676 nucleotide acids of the carboxyl end of nucleoprotein gene were amplified with reverse transcription-polymerase chain reaction（RT-PCR）,and then the PCR products were directly sequenced and analyzed.The phylogenetic tree was constructed based on 450nucleotide acids of the carboxyl end of nucleoprotein gene,and homological analysis was performed at nucleotide acid level and amino acid level.Results The isolate BJCY13026-2 was within the same genotype group of WHO genotype D₉ reference strain Victoria.AUS 12.99,the homology of nucleotide acid and amino acid was 95.8%and 96%respectively.Compared with other 23 genetype reference strains,the homology of nucleotide acid and amino acid were88.1%-95.6%and 90.7%-96.7%respectively.Compared with the Chinese measles vaccine strain Shanghai- 191,the homology of nucleotide acid and amino acid was 91.1%and 90.0%respectively.Compared with the major circulating measles strain genotype H_1a in China,the homology of nucleotide acid and amino acid was 89.4%and91.3%respectively.Conclusion Measles virus genotype D₉ was detected from an imported measles case in Beijing.更多还原