【摘要】 目的探讨葛根素对氧化应激状态下人视网膜色素上皮细胞(ARPE-19 cells)的保护作用。方法用200μmol/L过氧化氢(H2O2)诱导氧化应激,不同浓度葛根素作用于ARPE-19细胞24 h。应用比色法观察每组中超氧化物歧化酶(SOD)、谷胱甘肽(GSH)及丙二醛(MDA)含量的变化。结果 H2O2能够降低SOD活性,降低GSH含量,并且增加MDA含量,应用葛根素剂量依赖性地减少了以上效应。结论葛根素能够通过调节细胞内抗氧化标记物,保护人视网膜色素上皮细胞抵御H2O2诱导的氧化应激。更多还原

【Abstract】 Objective To investigate the protective effect of puerarin on ARPE-19 cells against oxidative stress. Methods ARPE-19 cells exposed to 200 μmol / L hydrogen peroxide( H2O2) were incubated with various concentrations of puerarin for 24 hours. The activity of the superoxide dismutase( SOD),and the change of the content of glutathion( GSH) and malonaldehyde( MDA) in each group were observed by chromatometry. Results H2O2decreased the activity of SOD,decreased the content of GSH and increased the content of MDA,that were reduced with different concentrations of puerarin in a dose-dependent manner. Conclusion Puerarin could protect ARPE-19 cells against H2O2-induced oxidative stress through modulation of intracellular antioxidant biomarkers.更多还原