【摘要】 阳离子端基肽类脂作为一种重要的非病毒基因载体,在基因转运方面具有广阔的应用前景。类脂结构是转染效率的主要因素,亲水头部起着脂质体与DNA、脂质体-DNA复合物与细胞膜或与细胞内其他组分相互结合的作用。基因表达效率与阳离子类脂的端基肽头部有关。而且,类脂的毒性也受到端基肽头部的影响。在近年来阳离子端基肽类脂的研究基础上,从阳离子端基肽类脂头部的构建进行了论述,概述了端基肽头部的种类及其基因转运效率,展望了阳离子端基肽类脂的发展方向。更多还原

【Abstract】 As an important non-viral gene vector,polypeptide cationic liposome has shown a potential application in disease treatment and gene transfer.The structure of cationic lipids is a major factor for their transfer activity.The structure of the hydrophilic domain plays a important role on the mutually combination of liposomes with DNA and liposomes/DNA lipoplex with cell membrane.The transfer efficiency is related to the terminal peptide headgroup.Also,the toxicity of the lipids is influenced by the terminal peptide headgroup.Based on the study of recent years,the latest development of polypeptide cationic liposome for gene delivery,including the structure design of polypeptide lipid,liposomal preparation and its application in the field of gene transfer is reviewed and some suggestions on the refearch trend of polypeptide cationic liposome for gene delivery are given as well.更多还原