【摘要】 目的:检测正常糖耐量(NGT)人群及新诊断2型糖尿病(T2DM)患者血浆分泌型卷曲相关蛋白5(SFRP5)水平,探讨SFRP5与体脂、糖脂代谢、胰岛素抵抗等因素的关系。方法:招募58名NGT且体重正常人群及54例新诊断T2DM患者,依据2000年WHO推荐的亚洲成年人肥胖诊断标准将体重指数(BMI)<25kg/m2定义为正常体重。采用ELISA法测定血浆SFRP5含量,稳态模型评估胰岛素抵抗指数(HOMA-IR)。结果:①T2DM组血浆SFRP5水平明显低于NGT组[(9.74±3.25)比(13.56±3.49)μg/L,P<0.05];②相关分析显示血浆SFRP5水平与BMI、腰臀比(WHR)、腰围(WC)、空腹血糖(FPG)、糖化血红蛋白(HbA1c)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、甘油三酯(TG)、丙氨酸氨基转移酶(ALT)及γ-谷氨酰转移酶(γ-GT)均呈负相关(P<0.05);③多元逐步回归分析显示HOMA-IR、BMI是血浆SFRP5水平的独立影响因素(β1=-0.429、β2=-0.236,均P<0.05)。结论:SFRP5在T2DM患者中表达明显降低,可能参与了T2DM发病过程。更多还原

【Abstract】 Objective:To detect plasma secreted frizzled-related protein(SFRP)5level in normal glucose tolerance(NGT)and newly diagnosed type 2diabetes mellitus(T2DM)subjects and to investigate the relationships between plasma SFRP5level and body fat parameters,glucolipid metabolism and insulin resistance.Methods:Fifty-eight NGT subjects with normal weight and Fifty-four T2DM patients were enrolled.Normal weight was defined as body mass index(BMI)<25kg/m2according to the World Health Organization-Western Pacfic Region diagnostic criteria(2000).Plasma SFRP5was detected by ELISA and insulin resistance index(IR)was assessed by homeostasis model assessment(HOMA).Results:Plasma SFRP5level in T2DM group was significantly lower than in NGT group([9.74±3.25]vs[13.56±3.49]μg/L,P<0.05);Correlation analysis showed that plasma SFRP5level was negatively correlated with BMI,waist hip ratio(WHR),waist circumference(WC),fasting plasma glucose(FPG),glycosylated hemoglobin(HbA1c),fasting insulin(FINS),homeostasis model assessment of insulin resistance(HOMAIR),triglyceride(TG),alanine aminotransferase(ALT),and Gamma-glutamyl transferase(γ-GT)(P<0.05).Multiple linear regression showed that HOMA-IR and BMI were independentrelated factors influencing the level of plasma SFRP5(β1=-0.429,β2=-0.236,P<0.05).Conclusion:Plasma SFRP5level was decreased in T2DM subjects and it may contribute to the pathophysiology of T2DM.更多还原